几种检测活性氧的荧光分子探针的合成及应用

发布时间：2017-12-28 10:43

本文关键词：几种检测活性氧的荧光分子探针的合成及应用　出处：《湖南师范大学》2016年硕士论文　论文类型：学位论文

【摘要】：荧光分子探针具有选择性好,灵敏度高,操作简单,费时少,生物相容性好等优点,被广泛应用于生物科学、基础医学和环境监测等领域。活性氧物种不仅在各种各样的生理和病理过程中扮演着重要角色,而且还和生物体内多种酶有着密切联系。本论文设计合成了三种基于活性氧检测的荧光分子探针分别检测次氯酸根,葡萄糖和乙酰胆碱/农药,并对其应用进行了研究。主要工作如下:(1)设计合成了一种简单的含有7-硝基苯并-2-氧杂-1,3-二唑和硒代吗啉的荧光探针1。该探针能够高选择性、高灵敏性和快速(10s)识别水溶液中的次氯酸根,检测下限可以达到3.3 nM。此外,该探针在谷胱甘肽-次氯酸根作用下可循环监测次氯酸根,用于细胞中次氯酸根的检测也得到了满意的结果。(2)设计合成了一种能荧光-比色检测双氧水的含有正己酸和硼酸酯结构的萘酰亚胺衍生物2。基于酶解葡萄糖间接检测双氧水可达到检测水相中葡萄糖的目的。实验研究表明,该探针具有选择性好、灵敏度高,检测下限低(0.3μM)的特点。此外,该探针能够很好地检测血清样本中的葡萄糖,这表明该探针可用于实际样品的测试。(3)设计合成了一种新型检测双氧水的水溶性荧光探针3,4-硼酸-N-(2-吡啶甲基)-1,8-萘酰亚胺乙基季铵盐。该探针本身荧光很弱,与催化氧化乙酰胆碱产生的过氧化氢作用后会产生荧光产物,出现"turn-on"的荧光信号,实现对乙酰胆碱的检测。研究表明,该方法对乙酰胆碱具有选择性好、灵敏度高、操作简单和费时少的特点,同时利用该原理还能实现对食品中的农药残留的检测,具有很高的应用价值。
[Abstract]:Fluorescent molecular probes have many advantages, such as good selectivity, high sensitivity, simple operation, less time and good biocompatibility. They are widely used in biological science, basic medicine and environmental monitoring. Active oxygen species not only play an important role in a variety of physiological and pathological processes, but also have a close relationship with various enzymes in the organism. Three kinds of fluorescent molecular probes based on reactive oxygen detection were designed and synthesized for the detection of chlorate, glucose and acetylcholine / pesticides respectively, and their applications were studied. The main work is as follows: (1) a simple fluorescent probe 1, which contains 7- nitrobenzene and -2- oxo -1,3- two azoles and seleno morpholine, is designed and synthesized. The probe can be highly selective, sensitive and rapid (10s) to identify the perchlorate in the aqueous solution, and the detection limit can reach 3.3 nM. In addition, the probe can be used to monitor hypochlorite under the action of glutathione - hypochlorite, and the detection of hypochlorite in cells is also satisfactory. (2) a naphthimide derivative 2 with hexanic acid and borate structure was designed and synthesized by the fluorescence - colorimetric detection of hydrogen peroxide. The purpose of detecting glucose in water phase can be achieved by the indirect detection of hydrogen peroxide based on enzymatic hydrolysis of glucose. The experimental study shows that the probe has the characteristics of good selectivity, high sensitivity and low detection limit (0.3 u M). In addition, the probe can be used to detect the glucose in the serum samples well, which indicates that the probe can be used for the test of the actual sample. (3) a new water-soluble fluorescent probe, 3,4- boric acid -N- (2- pyridyl methyl), -1,8- naphthalimide ethyl quaternary ammonium salt, was designed and synthesized. The probe itself has very weak fluorescence. It will produce fluorescence products after the action of hydrogen peroxide catalyzed by the oxidation of acetylcholine. The fluorescence signal of "turn-on" will appear, and the detection of acetylcholine can be realized. The research shows that the method is selective, sensitive, simple and time-consuming for acetylcholine, and it can also be used to detect pesticide residues in foods, and has high application value.
【学位授予单位】：湖南师范大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：O657.3

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