脯氨酸修饰二氧化硅的制备及糖肽富集研究

发布时间：2017-12-31 19:34

本文关键词：脯氨酸修饰二氧化硅的制备及糖肽富集研究　出处：《武汉理工大学学报》2016年06期 　论文类型：期刊论文

【摘要】：将丙烯酰化脯氨酸(acrylamide proline,AA-Pro)通过原子转移自由基聚合(ATRP)成功地固定到介孔二氧化硅上,构筑了一种新型色谱材料,并应用于糖肽的高效分离与富集。通过一系列测试方法证明丙烯酰化脯氨酸成功接枝在硅球上。同时,在糖肽分离富集测试中,该材料表现出良好的效果,从Fetuin酶解液中共获得15条糖肽,与富集前相比,检测到糖肽的个数增多,且信号强度增强。实验证明丙烯酰化脯氨酸修饰介孔二氧化硅可用于糖肽的分离富集中,同时为开发糖肽富集材料提供了一个新途径。
[Abstract]:The acryloyl proline (acrylamide proline, AA-Pro) via atom transfer radical polymerization (ATRP) was successfully immobilized onto the mesoporous silica, and build a new type of chromatographic materials, and efficient separation and enrichment of glycopeptides applied. Through a series of test methods that acryloyl proline was successfully grafted onto the silica spheres. At the same time, the separation and enrichment of glycopeptides test, the material showed good results, the solution obtained 15 glycopeptides from Fetuin enzyme, compared with the detected number of pre enrichment, glycopeptides increased, and enhanced signal intensity. Experiments prove that the acryloyl proline modified mesoporous silica can be used for the separation and enrichment of glycopeptides concentration at the same time, it provides a new way for the development of glycopeptide enrichment materials.

【作者单位】：武汉理工大学材料复合新技术国家重点实验室;
【基金】：国家自然科学基金(51533007)
【分类号】：O613.72;O652.6;O629.7
【正文快照】： 蛋白质的糖基化修饰作为蛋白质翻译后修饰的一种重要类型[1],具有极其重要的意义。糖基化修饰影响蛋白质的三维结构,同时对其功能有着重大的影响。无论是细胞间识别及信号传递,还是物质代谢中均有糖基化蛋白的参与。同时,蛋白质糖基化修饰的异常被认为与糖尿病、恶性肿瘤及老

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