基于GC-MS及电子鼻的云南红茶香气成分的研究

发布时间：2018-01-14 07:10

本文关键词：基于GC-MS及电子鼻的云南红茶香气成分的研究　出处：《天津科技大学》2016年硕士论文　论文类型：学位论文

【摘要】：本研究采用气相色谱-质谱联用(GC-MS)及电子鼻对云南红茶的香气成分的进行了研究。主要研究内容如下:1不同极性色谱柱在云南红茶香气成分分析中的对比研究比较了两种不同极性的色谱柱对云南红茶香气化合物分离效果的差异,HP-5MS以及CP-Wax两种色谱柱对云南红茶香气成分的分离效果均表现良好,但对不同种类化合物的分离效果存在一定的差异,色谱柱HP-5MS更适合用于云南红茶主要香气及整体香气成分分析。2电子鼻结合GC-MS在云南红茶香气分析中的应用建立了电子鼻对云南红茶香气分析的前处理以及分析方法,46种不同的云南红茶分为A、B、C三大类。通过对五种萃取头的灵敏性进行考察,确定了 75 μmCAR/PDMS萃取头更适合用于对云南红茶整体香气的综合分析。对三类云南红茶的代表性茶样进行GC-MS分析,结果表明,B类和C类云南红茶均以芳樟醇为主,而A类云南红茶则以香叶醇为主,此外,B类云南红茶中的水杨酸甲酯的百分比含量明显高于其他两类云南红茶。造成A类、B类和C类云南红茶香气差异的香气化合物主要为芳樟醇及芳樟醇氧化物、香叶醇、水杨酸甲酯等,除此之外还包括二氢芳樟醇、α-萜品醇、橙花叔醇、苯甲醛等主要香气成分以及其他一些含量较低的香气化合物,云南红茶的主要香气成分对云南红茶的分类结果有较大的影响。3云南红茶主要香气成分的定量分析方法研究根据累积峰面积标准化值考察五种不同的萃取头对云南红茶主要香气成分的萃取效率,结果表明,50/30μm DVB/CAR/PDMS萃取、65μm PDMS/DVB萃取头、75 μm CAR/PDMS萃取头分别对云南红茶的主要香气成分表现出较高的萃取效率。通过单因素实验对搅拌转速、萃取时间、萃取温度以及茶水比等影响萃取头萃取效率的萃取因素分别进行了优化,得到了 50/30 μm DVB/CAR/PDMS萃取头、65μm PDMS/DVB萃取头以及75μmCAR/PDMS萃取头的最佳萃取条件。在最佳萃取条件下,分别对最佳萃取方法进行验证,目标化合物的线性相关系数(R2)在均在0.995-0.999之间,线性关系良好,检出限均小于20 ng/g,重复性和准确性良好,适合对云南红茶的主要香气成分进行定量分析。
[Abstract]:Gas chromatography-mass spectrometry (GC-MS) was used in this study. The aroma components of Yunnan black tea were studied by electronic nose. The main contents were as follows:. 1Contrastive study of different Polar chromatographic columns in the Analysis of aroma components of Yunnan Black Tea the results of the separation of aroma compounds from Yunnan Black Tea by two different polar columns were compared. HP-5MS and CP-Wax chromatographic columns showed good separation effect on aroma components of Yunnan black tea, but there were some differences in the separation of different kinds of compounds. The chromatographic column HP-5MS is more suitable for the analysis of the main aroma and the whole aroma components of Yunnan black tea. 2 the application of electronic nose combined with GC-MS in the aroma analysis of Yunnan black tea has been established. The effect of electronic nose on the aroma of Yunnan black tea was established. Preprocessing and analytical methods of analysis. Forty-six different kinds of black tea from Yunnan were divided into three groups of Acarinobium C. the sensitivity of the five extraction heads was investigated. The 75 渭 mCAR/PDMS extraction head was determined to be more suitable for the comprehensive analysis of the whole aroma of Yunnan black tea. The representative tea samples of three kinds of Yunnan black tea were analyzed by GC-MS, the results showed that. The linalool is the main component of the black tea of B and C, while the geraniol is the main part of the black tea of A. The percentage of methyl salicylate in the black tea of B was significantly higher than that of the other two kinds of black tea, and the main aroma compounds that caused the difference in aroma between class A, B and C were linalool and linalool oxide. In addition, it includes dihydrolinalool, 伪 -terpene alcohol, nerolflower tertiary alcohol, benzaldehyde and other aroma compounds with lower content. The main aroma components of Yunnan black tea have great influence on the classification results of Yunnan black tea. 3 quantitative analysis of the main aroma components of Yunnan black tea; study on five different extraction head pairs according to the standardized value of cumulative peak area. Extraction efficiency of main aroma components of Yunnan black tea. The results show that 50 / 30 渭 m DVB/CAR/PDMS extraction head is 65 渭 m PDMS/DVB. The 75 渭 m CAR/PDMS extraction head showed high extraction efficiency for the main aroma components of Yunnan black tea, and the stirring speed and extraction time were tested by single factor experiment. The extraction temperature and the ratio of tea to water were optimized, and the 50/30 渭 m DVB/CAR/PDMS extraction head was obtained. The optimum extraction conditions of 65 渭 m PDMS/DVB extraction head and 75 渭 mCAR/PDMS extraction head were studied. Under the optimum extraction conditions, the optimum extraction method was verified. The linear correlation coefficient of the target compounds is between 0.995-0.999, the linear relationship is good, the detection limit is less than 20 ng / g, the repeatability and accuracy are good. It is suitable for quantitative analysis of the main aroma components of Yunnan black tea.
【学位授予单位】：天津科技大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：O657.63;TS272.52

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