色氨酸—酪氨酸混合体系高压在线三维荧光特性的研究

发布时间：2018-01-14 06:11

本文关键词：色氨酸—酪氨酸混合体系高压在线三维荧光特性的研究　出处：《浙江工业大学》2016年硕士论文　论文类型：学位论文

【摘要】：海洋中氨基酸的相对浓度和分布规律是海洋碳、氮循环的重要指标。色氨酸(Trp)、酪氨酸(Tyr)具有的天然荧光,为其在海洋中的在线监测提供了一种独特的指纹示踪手段以及灵敏简便的检测方法—荧光分析法。荧光分子的荧光性质不仅与其自身的分子结构有关,还与所处环境的理化性质紧密相关,例如温度、压力、溶液酸度等。色氨酸、酪氨酸在海洋或其他自然水体中是同时存在的,海洋环境又是复杂多变的,因此,研究典型环境因素对色氨酸-酪氨酸混合体系荧光性质的影响是有实际意义和参考价值的。三维荧光光谱法因其信息量丰富、选择性好、灵敏度高等特点,可用于混合体系荧光性质的研究。论文在对荧光基本理论和三维荧光分析技术充分调研的基础上,提出采用三维荧光光谱结合参量化方法,共设计54组实验,分别研究pH值、重金属离子、静水压力对色氨酸-酪氨酸混合体系三维荧光光谱的影响。主要结论如下:(1)弱碱性范围内pH值的微小变化对该混合体系三维荧光光谱的谱图轮廓及指纹性质有着明显的影响。(2)Cu~(2+)、Ni~(2+)对该混合体系均有荧光猝灭效应,且猝灭程度随离子浓度的增加而增大,Cu~(2+)的猝灭效应大于Ni~(2+)。(3)压力对该混合体系的光谱位置及荧光强度都有影响,而且压力对荧光强度的增敏作用与溶液中金属离子浓度有关。以上有关结论对海洋氨基酸的在线监测及生物大分子的结构研究有一定的参考价值。
[Abstract]:The relative concentration and distribution of amino acids in the ocean is an important indicator of the carbon and nitrogen cycle in the ocean. It provides a unique fingerprint tracing method and a sensitive and simple detection method for on-line monitoring in the ocean. The fluorescence properties of fluorescent molecules are not only related to their own molecular structure. Also closely related to the physical and chemical properties of the environment, such as temperature, pressure, solution acidity and so on. Tryptophan and tyrosine exist simultaneously in the ocean or other natural water bodies, the marine environment is complex and changeable, therefore. It is of practical significance and reference value to study the influence of typical environmental factors on the fluorescence properties of tryptophan tyrosine mixed system. Three-dimensional fluorescence spectrometry is characterized by rich information, good selectivity and high sensitivity. It can be used to study the fluorescence properties of the mixed system. On the basis of the investigation of the basic theory of fluorescence and the technique of three-dimensional fluorescence analysis, a method of three-dimensional fluorescence spectrum combined with reference quantization is proposed to design 54 groups of experiments. The pH and heavy metal ions were studied. The effect of hydrostatic pressure on the three-dimensional fluorescence spectra of tryptophan tyrosine mixed system. The main conclusions are as follows: 1). The slight change of pH value in the range of weak alkalinity has obvious influence on the spectral profile and fingerprint properties of the three dimensional fluorescence spectra of the mixed system. Ni~(2) has fluorescence quenching effect on the mixed system, and the quenching degree increases with the increase of ion concentration. The quenching effect of Cu~(2 is greater than that of Ni~(2. 3) the pressure has an effect on the spectral position and fluorescence intensity of the mixed system. The sensitizing effect of pressure on fluorescence intensity is related to the concentration of metal ions in solution. The above conclusions have some reference value for the on-line monitoring of marine amino acids and the study of the structure of biological macromolecules.
【学位授予单位】：浙江工业大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：O657.3

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