野蔷薇中齐墩果酸和熊果酸的测定、提取及指纹图谱研究

发布时间：2018-01-28 14:03

  本文关键词： 野蔷薇 齐墩果酸 熊果酸 测定 提取 指纹图谱 毒性实验　出处：《新疆师范大学》2016年硕士论文　论文类型：学位论文

【摘要】：野蔷薇(Rosa Multiflora Thunb)属于蔷薇科蔷薇属植物,又名多花蔷薇,是一种重要的药食两用的植物资源,含有丰富营养物质,具有很高的研究价值。本文采用HPLC法对野蔷薇中齐墩果酸和熊果酸测定方法、提取及果实提取物的指纹图谱等较为系统地研究,并研究了野蔷薇总皂苷对昆明种小鼠的毒性。通过实验所得到的实验结果如下:1)野蔷薇中齐墩果酸和熊果酸分析方法的建立:采用HPLC法测定野蔷薇不同部位(根、茎、果实)中齐墩果酸和熊果酸的含量且比较了12批野蔷薇果实中两者含量。齐墩果酸和熊果酸在20 min内较好分离,其质量浓度分别为0.02468~0.2468 mg/m L和0.02523~0.2523 mg/mL时与峰面积线性关系良好,相关系数分别为0.9994和0.9996;平均回收率为分别为99.96%(RSD为0.74%)和100.03%(RSD为0.95%)。齐墩果酸和熊果酸均在果实中含量最高;库车县、阿图什市的野蔷薇果实中齐墩果酸和熊果酸的含量最多,可作为最适开发利用的固定来源。2)响应面法优化野蔷薇果实中齐墩果酸和熊果酸提取工艺研究:采用超声波提取法提取野蔷薇果实中齐墩果酸和熊果酸,以单因素试验为基础,结合响应面试验优化野蔷薇果实中齐墩果酸和熊果酸的提取工艺。齐墩果酸最佳提取工艺条件为:液料比16:1mL/g,提取温度41℃,提取时间37min;熊果酸最佳提取条件为:液料比17:1mL/g,提取温度40℃,提取时间38min。在此工艺条件下齐墩果酸和熊果酸实际得率分别为0.3981 mg/g和0.7170mg/g,与模型预测结果(0.4067mg/g和0.7311mg/g)基本相符。响应曲面法优化超声波提取野蔷薇果实中齐墩果酸和熊果酸的预测准确,操作简单,可以为野蔷薇果实中齐墩果酸和熊果酸的开发利用提供了参考数据。3)野蔷薇果实提取物的HPLC指纹图谱研究:采用高效液相色谱法研究不同产地野蔷薇果实提取物的指纹图谱。实验结果表明野蔷薇果实提取物的指纹图谱检出7个共有峰,但各批药材相同成分的含量差异较大,其精密度、稳定性、重复性均良好。该方法可为野蔷薇果实的质量控制提供参考。4)野蔷薇总皂苷的毒性研究:本研究为了评价野蔷薇总皂苷的毒性,对野蔷薇总皂苷进行毒性实验,从实验结果得出,该药总皂苷提取物LD505g/kg,属无毒化合物。
[Abstract]:Rosa Multiflora Thunb (Rosa Multiflora Thunb), also known as Rosa multiflorus, is an important medicinal and edible plant resource, which contains rich nutrients. In this paper, HPLC method was used to determine oleanolic acid and ursolic acid in rose, extraction and fingerprint of fruit extracts were studied systematically. The toxicity of total saponins of Rosa rose to Kunming mice was studied. The experimental results are as follows: 1). Establishment of analytical method for oleanolic acid and ursolic acid in wild rose: HPLC method was used to determine different parts (roots) of wild rose. The contents of oleanolic acid and ursolic acid in stems and fruits were compared and the contents of oleanolic acid and ursolic acid were compared in 12 batches of Rosa rugosa fruits. Oleanolic acid and ursolic acid were better separated within 20 min. The linear relationship between the concentration and peak area is good when the mass concentration is 0.02468 mg/mL and 0.02523 mg/mL, respectively. The correlation coefficients were 0.9994 and 0.9996, respectively. The average recoveries were 99.96 (RSD = 0.74) and 100.03 (RSD = 0.95), respectively. The contents of oleanolic acid and ursolic acid were the highest in the fruits. The content of oleanolic acid and ursolic acid in roserose fruit of Kuqa County, Atushi City was the highest. Optimization of extraction process of oleanolic acid and ursolic acid from roserose fruit by response surface method: extraction of oleanolic acid and ursolic acid from wild rose fruit by ultrasonic extraction. On the basis of single factor test and response surface test, the extraction process of oleanolic acid and ursolic acid from rose fruit was optimized. The optimum extraction conditions of oleanolic acid were as follows: the ratio of liquid to material was 16: 1 mL / g. The extraction temperature was 41 鈩，

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