加替沙星磁性表面分子印迹材料的制备及其识别特性的研究

发布时间：2018-01-30 23:49

本文关键词： 磁性二氧化硅加替沙星表面分子印迹聚合物吸附　出处：《分析化学》2016年10期 　论文类型：期刊论文

【摘要】：以γ-(甲基丙烯酰氧)丙基三甲氧基硅烷(MPS)修饰的磁性二氧化硅(Fe_3O_4@SiO_2)为载体,加替沙星(GTFX)为模板分子,采用表面印迹法制备磁性表面分子印迹聚合物(M-MIPs)。用透射电镜(TEM)及磁化强度分析(VSM)对此聚合物进行了表征。吸附实验和Scatchard分析结果表明,M-MIPs中存在特异性和非特异性两类结合位点。M-MIPs和磁性非印迹聚合物(M-NIPs)对GTFX的最大吸附容量分别为35.1和23.13 mg/g。M-MIPs对于环丙沙星(CPFX)、诺氟沙星(NFLX)、三聚氰胺(MEL)以及四环素(TC)的选择性系数k分别为2.43,5.18,6.61和12.99;M-MIPs相对M-NIPs的相对选择性系数k'分别为2.09,1.95,3.15和2.43,表明M-MIPs对GTFX具有良好的特异性识别能力。将此表面印迹材料用于牛奶中GTFX的分离富集,采用高效液相色谱法检测,回收率大于91.5%。
[Abstract]:纬-(methacrylol) propyl trimethoxy silane (MPS) modified magnetic silica and gatifloxacin GTFX were used as carrier and GTFX as template molecule. Preparation of Magnetic Surface molecularly imprinted Polymer (M-MIPsN) by Surface imprinting. Transmission Electron Microscopy (TEM) and Magnetization Analysis (VSM). The polymer was characterized by adsorption experiments and Scatchard analysis. There are two kinds of binding sites in M-MIPs. M-MIPs and magnetic non-imprinted polymers (M-NIPs). The maximum adsorption capacities of GTFX for ciprofloxacin were 35.1 and 23.13 mg/g.M-MIPs, respectively. The selectivity coefficients of norfloxacin (NFLX), melamine (MEL) and tetracycline (TCC) were 2.43 ~ 5.186.61 and 12.99, respectively. The relative selectivity coefficients of M-MIPs to M-NIPs are 2.09 ~ 1.95 ~ 3.15 and 2.43, respectively. The results showed that M-MIPs had good recognition ability for GTFX. The surface imprinted material was used for the separation and enrichment of GTFX in milk and was detected by high performance liquid chromatography (HPLC). The recovery was more than 91.5%.
【作者单位】：宁夏大学化学化工学院;北方民族大学化学与化学工程学院;
【基金】：国家自然科学基金(No.21565001)资助项目~~
【分类号】：O631.3
【正文快照】： 1引言加替沙星(Gatifloxacin,GTFX)是第四代喹诺酮类广谱抗菌药物,多用于治疗和防控人以及动物的感染性疾病。近年来,畜牧养殖中的不规范用药导致动物性食品中兽药残留超标的事件时有发生,对人类的健康造成了威胁[1],因此对动物性食品中包括喹诺酮类药物在内的药物残留的检测

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