液滴微流控系统在数字聚合酶链式反应中的应用研究进展

发布时间：2018-01-31 00:43

本文关键词： 数字聚合酶链式反应液滴微流控芯片基因检测基因测序评述　出处：《分析化学》2016年08期 　论文类型：期刊论文

【摘要】：数字聚合酶链式反应(PCR)技术近年来发展迅速。与以实时荧光定量PCR为代表的传统PCR技术相比,数字PCR技术显著提高了定量分析的精确度和灵敏度。数字PCR的快速发展与近年来微流控技术在数字PCR技术中的广泛应用有着密切的联系。早期的研究和商业化产品使用的是大规模集成流路微流控芯片,加工过程复杂且价格高昂。近年来,液滴微流控芯片被应用到数字PCR技术中,它可以在短时间内产生102~107个微液滴,每一个微液滴都是最多只含有一个目的基因片段的PCR反应器。PCR扩增后,通过对单个微液滴的观察计数,就可以获得绝对定量的分析数据。本文综述了不同种类的液滴微流控系统在数字PCR技术中的应用,以及液滴数字PCR微流控芯片在生物、医药、环境等领域的应用。
[Abstract]:Digital polymerase chain reaction (PCR) technology has been developed rapidly in recent years, compared with the traditional PCR technology represented by real-time fluorescent quantitative PCR. Digital PCR technology has greatly improved the accuracy and sensitivity of quantitative analysis. The rapid development of digital PCR is closely related to the wide application of microfluidic technology in digital PCR technology in recent years. Large scale integrated flow path microfluidic chips are used in commercial products. In recent years, droplet microfluidic chips have been applied to digital PCR technology, which can produce 102 ~ 107 microdroplets in a short time. Each microdroplet was amplified from a PCR reactor containing at most one fragment of the target gene and counted by observation of a single microdroplet. In this paper, the applications of different droplet microfluidic systems in digital PCR technology and droplet digital PCR microfluidic chips in biology and medicine are reviewed. The application of environment and other fields.
【作者单位】：北京化工大学机电工程学院;
【分类号】：O652.9
【正文快照】： 1引言早在1992年,已有学者提出了数字聚合酶链式反应(Digital PCR,d PCR)的概念[1],并且指出其在DNA的定量分析方面具有显著优势。但是初期的d PCR技术发展十分缓慢,这是由于如果使用传统的96孔板或者384孔板进行PCR扩增,需要数个多孔板同时进行扩增,不仅操作复杂,且实验样品

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