GC-MS法测定罗汉果中拟除虫菊酯农药残留量

发布时间：2018-01-31 10:05

本文关键词： 罗汉果拟除虫菊酯气相色谱-质谱法(GC-MS)　出处：《食品研究与开发》2017年23期 　论文类型：期刊论文

【摘要】：建立气质联用(Gas chromatography-mass spectrometry,GC-MS)测定罗汉果中6种拟除虫菊酯类农药的残留量方法。样品用石油醚-丙酮混合溶剂超声提后过固相萃取小柱净化,经HP-5MS色谱柱分离,以气相色谱质谱法进行检测,用外标法计算含量。结果表明:6种拟除虫菊酯类农药在0.1μg/mL~1.0μg/mL范围内呈现良好的线性关系,相关系数为0.999 1~0.999 9,检出限为0.139μg/kg~5.262μg/kg。样品中3个加标水平的回收率为83.5%~106.1%,相对标准偏差(RSD)为0.9%~6.5%。本试验所建立的方法操作简便,具有良好的回收率,精密度和灵敏度,符合检测要求,可用于罗汉果中6种拟除虫菊酯类农药残留的测定。
[Abstract]:To establish gas chromatography-mass spectrometry. Six pyrethroid pesticide residues in Siraitia grosvenori were determined by GC-MS.The samples were extracted by ultrasonic extraction with petroleum ether-acetone mixed solvent and purified by solid-phase extraction column. The samples were separated by HP-5MS chromatographic column. The content of pyrethroid was determined by gas chromatography-mass spectrometry and the content was calculated by external standard method. The results showed that the linear range of 6 pyrethroid pesticides was 0.1 渭 g / mL and 1.0 渭 g / mL. The correlation coefficient was 0.999 1 ~ 0.999 9. The detection limit was 0.139 渭 g 路kg ~ (-1) 路kg ~ (-1) 路kg ~ (-1) 路kg ~ (-1) 路kg ~ (-1) 路kg ~ (-1) 路kg ~ (-1) 路kg ~ (-1) 路kg ~ (-1). The relative standard deviation (RSD) is 0.90.The method established in this experiment is simple to operate, has good recovery, precision and sensitivity, and meets the requirements of detection. It can be used for the determination of 6 pyrethroids pesticide residues in Siraitia grosvenori.
【作者单位】：桂林医学院广西高校生药学重点实验室;中国有色桂林矿产地质研究院有限公司;
【基金】：广西科学研究与技术开发计划项目(桂科能1598025-18、桂科能14123006-21)
【分类号】：O657.63;TS207.53
【正文快照】： 罗汉果[Siraitia Grosvenorii(Swing le)C.Jeffrey]属葫芦科罗汉果属的植物,具有清肺止咳,抗菌消炎等功效,是广西桂北地区特色药材[1]。罗汉果除了药用外,大部分用来生产甜苷,用于食品甜味剂,比如罗汉果糖,因为其含有甜味素,甜度高,深受糖尿病、肥胖者的喜欢[2-3]。由于罗汉果

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