AMWFA法结合HELP法在中药复杂体系中的应用

发布时间：2018-01-31 10:09

本文关键词： 中药交互移动窗口因子分析法(AMWFA) 直观推导式演进特征投影法(HELP) 气相色谱-质谱　出处：《中南大学学报(自然科学版)》2017年06期 　论文类型：期刊论文

【摘要】：采用交互移动窗口因子分析法(AMWFA)对2个体系中的2组二维数据片段进行比较分析,得到2组二维数据片段中的共有组分;采用直观推导式演进特征投影法(HELP),利用二维矩阵数据包含的色谱/光谱信息和局部因子分析以分辨出各组分的纯色谱曲线和光谱,得到具有真实物理意义的唯一解。将AMWFA和HELP相结合,对中药复杂体系药对桂枝-荆芥进行解析。研究结果表明:AMWFA和HELP相结合能鉴定出药对与桂枝、荆芥的组分数分别为50,53和49个,各占挥发油总体积分数的91.87%,86.25%和92.86%;药对与桂枝共有26个组分,与荆芥共有22个组分,产生两单味药中没有的6个新成分;采用AMWFA法结合HELP法能最大程度且准确地完成中药复杂未知体系的鉴定。
[Abstract]:Two groups of 2D data fragments in two systems were compared and analyzed by the method of interactive moving window factor analysis (AMWFAA), and the common components of two groups of two-dimensional data fragments were obtained. The evolution feature projection method was used to distinguish the pure chromatographic curves and spectra of each component by using the chromatographic / spectral information contained in two-dimensional matrix data and local factor analysis. The unique solution with real physical meaning is obtained. AMWFA and HELP are combined. The complex system of traditional Chinese medicine (TCM) was used to analyze the components of Cinnamomum Cinnamomum and Schizonepeta thaliana. The results showed that the combination of HELP and HELP could identify the fractions of drug pair and Cinnamomum esculentum and Schizonepeta thaliana were 50 53 and 49 respectively. 86.25% and 92.86% of the total integral number of volatile oil were 91.87% and 92.86%, respectively. There were 26 components in the drug pair and cinnamon branch and 22 components in the Schizonepeta thaliana, which produced 6 new ingredients which were not found in the two drugs. AMWFA method combined with HELP method can be used to identify the complex unknown system of traditional Chinese medicine to the greatest extent and accurately.
【作者单位】：中南大学化学化工学院;长沙环境保护职业技术学院环境监测系;
【基金】：国家自然科学基金资助项目(20175036,20235020)~~
【分类号】：O652;R284
【正文快照】： 中药的化学成分非常复杂,单味药一般都含有大量化合物,复方药的成分则更复杂。随着分离以及鉴定技术的发展,新型的色谱-光谱二维联用仪器使用日益广泛,如气相色谱-质谱联用仪(GC-MS)、高效液相色谱-二极管阵列联用仪(HPLC-DAD)、毛细管电泳-二极管阵列联用仪(CE-DAD)等可以产

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