葡萄糖异构化对其糖聚合物蛋白识别功能的影响

发布时间：2018-02-04 01:59

  本文关键词： 糖聚合物 傒酰亚胺 光声成像 蛋白质　出处：《高分子学报》2017年12期 　论文类型：期刊论文

【摘要】：设计合成了具备良好的近红外吸收性能的傒酰亚胺大分子引发剂,并通过原子转移自由基聚合(ATRP)和后修饰方法制备了重复单元分别为一位羟基葡萄糖(1-Glc)和六位羟基葡萄糖(6-Glc)的糖聚合物.该聚合物在水溶液中能形成傒酰亚胺为核,糖聚合物为壳的纳米聚集体.通过动态光散射(DLS)、透射电子显微镜(TEM)和紫外-可见吸收光谱(UV-Vis)对聚合物在水溶液中的形貌、大小以及光学性能进行了分析表征,聚合物在650~750 nm有较好的近红外吸收特性;通过光声仪测试了聚合物的光声性能,结果表明傒酰亚胺糖聚合物具有较强的光声信号;为研究葡萄糖异构化对糖聚合物蛋白识别功能的影响,采用多种表征手段如浊度法、动态光散射研究了聚合物和凝集素半刀豆蛋白(concanavalin A,Con A)的结合.发现随着Con A凝集素的加入,PBI-1-Glc溶液的浊度和粒径有着明显的增加,而PBI-6-Glc则几乎没有变化;此外,还利用光声成像技术研究了糖聚合物与Con A之间的相互作用,Con A凝集素可使PBI-1-Glc聚合物的光声信号明显增加,此结果表明光声技术也可以有效地识别2种聚合物与凝集素之间的相互作用.实验结果显示,PBI-1-Glc聚合物与凝集素Con A有强的相互作用,而PBI-6-Glc聚合物则没有,这证实了单糖异构化在影响其与凝集素的特异性识别方面具有一定普适性.
[Abstract]:An imide macromolecular initiator with good near infrared absorption property was designed and synthesized. The repeat units were prepared by atom transfer radical polymerization (ATRP) and post modification, respectively. The repeat units were one hydroxyl glucose 1-Glc and six hydroxyl glucose 6-Glc, respectively. The polymer can form imide as core in aqueous solution. The morphology of the polymer in aqueous solution was investigated by dynamic light scattering (DLS), transmission electron microscopy (TEM) and UV-Vis-UV absorption spectroscopy (UV-Vis). The size and optical properties of the polymer were analyzed and characterized. The polymer has good near infrared absorption at 650 ~ 750 nm. The photoacoustic properties of the polymer were measured by photoacoustic instrument. The results show that the polymer has a strong photoacoustic signal. In order to study the effect of glucose isomerization on the recognition function of sugar polymer protein, many characterization methods such as turbidimetric method were used. Dynamic light scattering (DLS) was used to study the binding of polymer to concanavalin Aconcanavalin Con A. It was found that with the addition of Con A agglutinin. The turbidity and particle size of PBI-1-Glc solution increased obviously, but PBI-6-Glc had little change. In addition, the photoacoustic imaging technique was used to study the interaction between sugar polymer and Con A. The lectin of Con A can increase the photoacoustic signal of PBI-1-Glc polymer. The results show that the photoacoustic technique can also effectively identify the interaction between two kinds of polymers and lectins. The experimental results show that PBI-1-Glc polymer has strong interaction with lectin Con A. However, PBI-6-Glc polymers do not, which proves that monosaccharide isomerization has a certain universality in affecting its specific recognition with lectins.
【作者单位】： 南京工业大学江苏省柔性电子重点实验室先进材料研究院江苏先进生物与化学制造协同创新中心;
【基金】：国家重点基础研究发展计划(973计划,项目号2015CB932200) 国家自然科学基金(基金号21574064,61136003)资助项目
【分类号】：O631
【正文快照】： **通讯联系人,E-mail:wei-huang@njtech.edu.cn糖类化合物作为维持生命与健康的营养物质之一,不仅为生物体提供主要能源,而且还具备多种生物功能,其中糖-蛋白之间的特异性识别在细胞识别、细胞黏附、病菌感染、免疫反应等过程中发挥着重要的作用[1~8].糖异构现象广泛存在于糖，

本文编号：1488985