基于纸质微流控芯片的农药检测系统

发布时间：2018-02-24 20:13

本文关键词： 农药检测系统酶抑制法纸质微流控芯片酶电极电化学检测　出处：《农业机械学报》2017年05期 　论文类型：期刊论文

【摘要】：针对当前农药检测手段仪器复杂、成本昂贵等问题,提出了一种基于纸质微流控农药检测方法。设计了具有自动进样、混合反应、电化学检测等功能的纸质微流控芯片,采用石墨碳、Ag/AgCl材料以及结合化学交联法制备了环状结构的丝网印刷酶电极,并利用循环伏安法对制备的酶电极进行了电化学表征,构建了一套基于酶抑制法的集成酶电极纸质微流控农药检测系统。最后建立了酶抑制率与对硫磷浓度的数学模型,并测试了酶电极的性能。实验结果表明,酶电极具有良好的制备重复性、稳定性和线性度。抑制率与对硫磷浓度的负对数在1.0×10~(-7)~1.0×10~(-5)g/mL范围内呈良好的线性关系,线性回归方程为:I=158.82+21.11lg C,R~2为0.993,最低检出限为3.3×10~(-8)g/mL。所制备的酶电极微流控传感器抗干扰性较强,对对硫磷农药具有一定的选择性。加标回收率范围在95.8%~115.0%之间。
[Abstract]:A paper microfluidic chip with automatic sampling, mixed reaction and electrochemical detection was designed to solve the problems such as the complexity and high cost of the present pesticide detection methods, and a paper microfluidic detection method based on paper microfluidic was proposed. The ring structure screen printing enzyme electrode was prepared by graphite carbon mono AgCl material and chemical crosslinking method. The electrochemical characterization of the enzyme electrode was carried out by cyclic voltammetry. An integrated enzyme electrode paper microfluidic pesticide detection system based on enzyme inhibition method was constructed. At last, a mathematical model of enzyme inhibition rate and parathion concentration was established, and the performance of enzyme electrode was tested. The enzyme electrode has good reproducibility, stability and linearity. The negative logarithm of the inhibition rate and the concentration of parathion is linear in the range of 1.0 脳 10 ~ (-1) ~ (-7) ~ (-1) ~ (-1) ~ (-1) g / mL. The linear regression equation was: 1: Igna 158.82 21.11lg / g Rn2 = 0.993, and the lowest detection limit was 3.3 脳 10 ~ (-1) ~ (-8) g / mL 路mL ~ (-1). The enzyme electrode microfluidic sensor had strong anti-interference and selectivity to parathion pesticide. The recoveries of the enzyme electrode were in the range of 95.8% and 115.0%.
【作者单位】：江苏大学现代农业装备与技术教育部重点实验室;江苏大学电气信息工程学院;加州大学圣地亚哥分校信息理论与应用研究中心;江苏大学化工化学学院;
【基金】：“十二五”国家科技支撑计划项目(2014BAD08B03) 国家自然科学基金重点项目(61233006);国家自然科学基金项目(31671584) 江苏省农业科技自主创新资金项目(CXC1571033-02) 江苏省高校优势学科建设工程项目(苏财教(2014)37号)
【分类号】：O657.1;TQ450.7

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