HPLC-DAD-FLD同时测定柑橘果汁中12种多甲氧基黄酮和香豆素类物质

发布时间：2018-02-24 21:27

  本文关键词： 高效液相色谱 荧光检测 柑橘果汁 多甲氧基黄酮 香豆素　出处：《食品科学》2017年20期 　论文类型：期刊论文

【摘要】：建立高效液相色谱-串联二极管阵列检测器和荧光检测器(high performance liquid chromatography-diode array detector-fluorescence detector,HPLC-DAD-FLD)同时检测柑橘果汁中12种多甲氧基黄酮、香豆素及呋喃香豆素类物质的方法。以0.01%磷酸、甲醇、乙腈组成三元流动相进行梯度洗脱,12种物质在30 min内实现基线分离。利用DAD和FLD获得各物质的紫外和荧光光谱信息,将柑橘果汁样品组分的光谱与之比对,并结合色谱保留时间,实现样品成分的定性分析。分别以320 nm和450 nm为紫外和荧光的检测波长,研究该方法的定量性能。结果显示:标准曲线线性关系良好;荧光定量限低至μg/L级,可作为紫外定量的重要补充手段;果汁回收率的紫外和荧光检测值分别为95.2%~104.8%和94.5%~103.5%。该方法对样品定性、定量分析准确,适合柑橘多甲氧基黄酮、香豆素等能发射荧光物质的检测。
[Abstract]:A method for simultaneous determination of 12 polymethoxyflavones, coumarins and furancoumarins in citrus juice by high performance liquid chromatography-diode array detector-fluorescence detector high performance liquid chromatography-diode array detector-fluorescence detector and fluorescence detector was established. A ternary mobile phase consisting of acetonitrile was used to carry out gradient elution of 12 substances in a baseline within 30 min. The UV and fluorescence spectra of each substance were obtained by DAD and FLD, and the spectra of citrus juice samples were compared with each other. The quantitative properties of the method were studied by using 320 nm and 450 nm as the detection wavelengths of UV and fluorescence, respectively. The results showed that the linear relationship of the standard curve was good. The fluorescence quantitative limit is as low as 渭 g / L and can be used as an important supplementary method for UV quantification. The UV and fluorescence detection values of the recovery rate of juice are 95.2% 104.8% and 94.5% 103.5%, respectively. This method is accurate for the qualitative and quantitative analysis of the samples and is suitable for citrus polymethoxy flavonoids. Coumarin and other emission fluorescent substances detection.
【作者单位】： 西南大学柑桔研究所中国农业科学院柑桔研究所;重庆第二师范学院重庆市功能性食品协同创新中心重庆市功能性食品工程技术研究中心;西南大学食品科学学院;重庆第二师范学院生物与化学工程学院;
【基金】：公益性行业(农业)科研专项(201503142-12) 重庆市功能性食品协同创新中心建设项目(167001);重庆市功能性食品协同创新中心科研项目(ccicff-rp-201604) 中央高校基本科研业务费专项(XDJK2016E048) 重庆市统筹城乡教师教育研究中心2015年工作室项目(JDGZS201507) 重庆第二师范学院2016年校级大学生创新创业训练计划项目(201605)
【分类号】：O657;TS255.44
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