基于金纳米粒子放大的毛细管电泳分析研究

发布时间：2018-03-02 02:20

本文关键词： 毛细管电泳胶束电动毛细管色谱纳米材料西甲鱼毒苯胺对甲苯胺　出处：《青岛科技大学》2017年硕士论文　论文类型：学位论文

【摘要】：本文主要研究了纳米材料在毛细管电泳在线富集方法上的应用,由于纳米材料具有生物相容性好、比表面积大、物理化学性质稳定等特点,于毛细管在线富集方法上具有显著作用。通过对纳米材料的应用,富集效果显著提高。本文共分为三个章节:第一章,主要对毛细管电泳技术进行了介绍。首先,讨论了毛细管电泳的发展及应用,重点涉及毛细管电泳的几种分离模式;然后,对毛细管电泳在线富集技术的发展进行了概括;其次,展望国内外毛细管电泳研究现状,对一些在毛细管电泳发展过程中具有重要作用的创新进行了介绍;最后,介绍了纳米材料在毛细管电泳上的应用,分别列举了几种具有重要特性的功能性纳米材料以及在毛细管电泳分离方法上所起到的作用。第二章,基于外部磁场和纳米金放大的毛细管电泳免疫分析超灵敏在线快速检测雪卡鱼毒。本章节提出了一种基于毛细管电泳分离与电化学免疫分析联用的快速灵敏在线检测CTX3C的方法。在这种三明治夹心结构的的免疫实验中,将修饰有CTX3C抗体的磁性纳米材料作为探针,同时将辣根过氧化物酶以及CTX3C抗体修饰到纳米金颗粒表面上作为识别因子。通过在毛细管进样口外部设置一个外部磁场使免疫复合物在线合成得以实现。与传统的高效液相色谱-质谱以及酶联免疫反应方法相比,这种采用纳米金作为多酶载体、基于外部磁场在线形成三明治结构的免疫复合物的方法具有灵敏度高、分析时间短等特点。本方法检测CTX3C的线性范围是0.6-150 ng·L-1,相关系数是0.9948,而最低检出限达到了0.09ng·L-1。本方法具有良好的稳定性和重现性,而且能够用于鱼类样品中鱼肉素的定量检测。第三章,基于纳米金信号放大的胶束电动毛细管色谱法在线检测苯胺和对甲苯胺。本实验提出了一种胶束电动毛细管色谱在线一次富集与纳米金二次信号放大联用的检测方法,用于快速在线检测苯胺和对甲苯胺。首先是在静电作用下,将带有正电荷的样品聚集到带有负电荷的胶团表面,形成带有正电荷的一次聚集体;然后在毛细管中引入带有负电荷的纳米金,同样在分子间静电作用下,带有正电荷的胶团聚集到纳米金颗粒表面,达到检测信号二次放大的目的。这种用于在线检测苯胺和对甲苯胺的方法,具有灵敏度高、操作简单、稳定性好等优点。苯胺和对甲苯胺的最低检出限分别达到了0.114×10-9 mol·L-1和2.656×10-9 mol·L-1。与毛细管区带电泳以及单纯的胶束电动毛细管色谱法相比,本方法用于检测苯胺和对甲苯的灵敏度分别提高了13421倍、6740倍和972倍、361倍。
[Abstract]:In this paper, the application of nanomaterials in the on-line enrichment of capillary electrophoresis was studied. Due to their good biocompatibility, large specific surface area, stable physical and chemical properties, etc. This paper is divided into three chapters: the first chapter mainly introduces the capillary electrophoresis technology. This paper discusses the development and application of capillary electrophoresis, focusing on several separation modes of capillary electrophoresis. Then, the development of capillary electrophoresis online enrichment technology is summarized. Secondly, the present situation of capillary electrophoresis research at home and abroad is prospected. Some innovations that play an important role in the development of capillary electrophoresis are introduced. Finally, the application of nanomaterials in capillary electrophoresis is introduced. Several functional nanomaterials with important properties and their roles in capillary electrophoresis separation are listed respectively. The detection of ciguatera venom by capillary electrophoresis based on external magnetic field and gold nanoparticles amplification is very sensitive and on line. In this chapter, a fast sensitive on-line method based on capillary electrophoresis separation and electrochemical immunoassay is proposed. A method for detecting CTX3C. In an immunological test of this sandwich sandwich structure, Using magnetic nanomaterials modified with CTX3C antibodies as probes, At the same time, horseradish peroxidase and CTX3C antibody were modified on the surface of gold nanoparticles as recognition factors. By setting an external magnetic field outside the capillary injection port, the on-line synthesis of immune complexes was realized. Compared with HPLC-MS and enzyme-linked immunosorbent assay (Elisa), This method, which uses nano-gold as multi-enzyme carrier and forms sandwich structure immune complex based on external magnetic field, has high sensitivity. The linear range of this method is 0.6-150 ng 路L ~ (-1), the correlation coefficient is 0.9948, and the minimum detection limit is 0.09 ng 路L ~ (-1). The method has good stability and reproducibility. And it can be used for quantitative determination of fish meat in fish samples. Micellar Electrokinetic Capillary Chromatography (MECC) based on nanoscale gold signal amplification was used to detect aniline and p-toluidine on line. In this paper, a new method for the on-line detection of aniline and p-toluidine by micellar electrokinetic capillary chromatography was proposed. It is used for rapid on-line detection of aniline and p-toluidine. Firstly, under electrostatic action, samples with positive charge are gathered on the surface of the micelle with negative charge to form a positive charge aggregate. Then the negatively charged nanocrystalline gold is introduced into the capillary, and the positively charged micelles gather on the surface of the nanoparticles under the same intermolecular electrostatic action. The method used for on-line detection of aniline and p-toluidine has high sensitivity and simple operation. The minimum detection limits of aniline and p-toluidine were 0.114 脳 10 ~ (-9) mol 路L ~ (-1) and 2.656 脳 10 ~ (-9) mol 路L ~ (-1), respectively, compared with capillary zone electrophoresis and micellar electrokinetic capillary chromatography. The sensitivity of this method for the determination of aniline and toluene was increased by 13421 times, 6740 times and 972 times, respectively.
【学位授予单位】：青岛科技大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：O658.9

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