涡旋辅助液液微萃取结合GC-MS法检测67种白酒中四甲基吡嗪、4-甲基愈创木酚和4-乙基愈创木酚

发布时间：2018-03-02 14:50

本文选题：白酒　切入点：四甲基吡嗪　出处：《食品科学》2017年18期 　论文类型：期刊论文

【摘要】：采用涡旋辅助液液微萃取结合气相色谱-质谱(gas chromatography-mass spectrometry,GC-MS)法对67种白酒中四甲基吡嗪、4-甲基愈创木酚和4-乙基愈创木酚进行检测。酒样经二氯甲烷提取,涡旋辅助萃取1 min,6 000 r/min离心5 min,然后采用GC-MS进行分析。结果表明,四甲基吡嗪、4-甲基愈创木酚和4-乙基愈创木酚分别在质量浓度为0.5~2 500、1.0~2 500、1.0~2 500μg/L的范围内具有较好的线性关系(R20.99);检出限分别为0.25、0.5、0.5μg/L;不同添加水平的平均回收率为80.1%~88.0%。对67种白酒含量分析表明,所有样本都含有四甲基吡嗪,含量范围为1.5~2 434.3μg/L;51种样本中含有4-甲基愈创木酚,含量范围为2.8~1 709.0μg/L;53种样本中含有4-乙基愈创木酚,含量范围为1.3~1 167.5μg/L。白酒酿造原料、工艺的差异可能是造成其健康因子含量不同的潜在因素。
[Abstract]:Tetramethylpyrazine 4-methylguaiacol and 4-ethyl guaiacol in 67 kinds of liquor were determined by vortex assisted liquid-liquid microextraction and gas chromatography-mass spectrometry (chromatography-mass) GC-MS.The samples were extracted by dichloromethane. Vortex-assisted extraction was centrifuged for 5 mins with 1 min-6 000 r / min, and then analyzed by GC-MS. The results showed that, Tetramethylpyrazine 4-methylguaiacol and 4-ethylguaiacol have a good linear relationship in the concentration range of 0.5 ~ 2 500 ~ (-1) C ~ (-1) ~ (-1) ~ (-1) C ~ (-1) C ~ (2 500) C ~ (2 500) 渭 g / L, the detection limit is 0.25 渭 g / L, the detection limit is 0.25 渭 g / L, and the average recovery at different levels is 80.1% ~ 88.0 路L ~ (-1). 67 species of guaiacol have been tested for 67 species. The analysis of liquor content showed that, All the samples contained tetramethylpyrazine, the content range was 1.5 ~ 2443. 3 渭 g / L ~ (-1) 渭 g 路L ~ (-1) L ~ (-1). In 51 samples, 4-methylguaiacol was found in 51 samples, and in 53 samples with a concentration range of 2.8 ~ 179.0 渭 g 路L ~ (-1) 渭 g 路L ~ (-1), the content of 4-ethyl guaiacol was 1.3N ~ (11) 167.5 渭 g / L ~ (-1) L 路L ~ (-1). The difference in process may be a potential factor for different health factors.
【作者单位】：天津科技大学食品工程与生物技术学院;食品质量与安全北京实验室(北京工商大学);泸州老窖股份有限公司;
【基金】：国家自然科学基金青年科学基金项目(31601556) “十三五”国家重点研发计划重点专项(2016YFD0400501) 四川省科技计划项目(2017JZ0012)
【分类号】：O657.63;TS262.3

【相似文献】