新型近红外荧光分子探针的设计合成及其在细胞成像中的应用

发布时间：2018-03-04 08:31

本文选题：荧光传感器　切入点：近红外荧光探针　出处：《中国科学技术大学》2017年硕士论文　论文类型：学位论文

【摘要】：重金属元素是一类常见的环境污染物,在饮用水和食品安全等方面需对其含量进行严格监控。其中铜是重金属元素中一类特殊的存在,它是维持人体健康必要的微量元素,在人体组织更新代谢及维持神经系统正常运转等方面具有重要作用。人体内铜的代谢异常会引起诸多病症,例如Alzheimer症、Parkinson症、Wilson疾病等。因此发展一种具有高度灵敏性的铜离子快速检测方法是一项非常有价值的工作。荧光检测法由于其具有灵敏性高,选择性好,设备价廉,操作简单,能够实现快速检测等优点,近年来引起人们的持续关注,广泛应用在重金属检测方面。目前,已经开发出包括量子点、金属团簇、碳点、有机染料等不同材料的荧光传感器,用于铜离子的荧光检测。其中,近红外荧光传感器具有对细胞更小的光损伤和较高的组织穿透性,能够克服生物组织背景荧光的干扰,特别适用于细胞成像方面。本文采用具有近红外发射的半花菁衍生物有机染料实现了对二价铜离子(Cu2+)的高选择性及灵敏性检测,并用其进行了细胞内Cu2+成像。本论文分为两章,每章具体内容如下:第一章:首先对荧光分析法进行了系统阐述,包括基本原理和检测机理;然后针对不同的机理,详述了针对Cu2+的荧光分析法的研究现状;接着对近红外荧光技术进行了阐述,并对不同近红外荧光材料及其在生物领域内的应用进行了概述;最后提出了论文选题及主要研究内容。第二章:介绍了具有近红外荧光发射的基于半花菁结构的新型有机荧光传感器(XL-Cu)的制备并对Cu2+进行了检测。该探针主要通过自身的邻羟基咪唑结构与铜离子进行动态配位,并对分子自身的半花菁荧光团产生影响,从而使半花菁部分的荧光降低。依照此现象,开发了一种检测Cu2+的新方法,该方法以荧光信号的变化为基础,具有高度的选择性,在水溶液中检测限可达14nM(R2=0.9994)。另外,该探针的最大发射峰位于近红外区域,对生物组织具有较小的光损伤和更深的穿透性,同时,其细胞毒性较低,因此适用于细胞成像。
[Abstract]:Heavy metal elements are a kind of common environmental pollutants, which need to be strictly monitored in drinking water and food safety, among which copper is a special kind of heavy metal elements, and it is a necessary trace element to maintain human health. It plays an important role in the regeneration and metabolism of human tissues and in maintaining the normal functioning of the nervous system. Abnormal copper metabolism in the human body can cause many diseases. For example, Parkinson's disease, Wilson's disease, etc. Therefore, it is very valuable to develop a highly sensitive method for the rapid detection of copper ions. The fluorescence detection method is simple because of its high sensitivity, good selectivity, cheap equipment and simple operation. It has attracted people's attention in recent years and has been widely used in heavy metal detection. At present, fluorescent sensors including quantum dots, metal clusters, carbon dots, organic dyes and other materials have been developed. The near infrared fluorescence sensor has smaller light damage to cells and higher tissue penetration, which can overcome the interference of background fluorescence of biological tissue. It is especially suitable for cell imaging. In this paper, the highly selective and sensitive detection of copper ion (Cu2) with half-cyanine derivatives with near infrared emission has been achieved. This paper is divided into two chapters. Each chapter is as follows: chapter one: firstly, the basic principle and detection mechanism of fluorescence analysis are described systematically, and then the different mechanisms are discussed. In this paper, the research status of fluorescence analysis for Cu2 is described in detail, and then the near infrared fluorescence technology is described, and the applications of different near infrared fluorescent materials in biological field are summarized. Chapter 2: the preparation of a novel organic fluorescence sensor with near-infrared fluorescence emission based on semi-cyanine structure and the detection of Cu2 are introduced. The structure of o-hydroxy imidazole is dynamically coordinated with copper ion. A new method for the detection of Cu2 is developed, which is based on the change of fluorescence signal and has high selectivity. In addition, the maximum emission peak of the probe is located in the near infrared region, which has less light damage and deeper penetration to biological tissue, and its cytotoxicity is low, so it is suitable for cell imaging.
【学位授予单位】：中国科学技术大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：O657.3

【参考文献】