内生真菌绿色生态法合成纳米银的研究

发布时间：2018-03-04 11:47

  本文选题：内生真菌　切入点：禾谷镰刀菌　出处：《现代食品科技》2017年01期 　论文类型：期刊论文

【摘要】：从石斛中分离得到一株内生真菌DO-1,该菌株经鉴定为禾谷镰刀菌(Fusarium graminearum),其菌体在去离子水中浸泡后分泌的代谢产物与硝酸银作用后,能还原银离子产生具有抗菌作用的纳米银粒子。以440~450 nm范围内纳米银的特征性等离子吸收峰以及产物抑菌活性为指标,研究光照条件、温度以及p H条件对制备纳米银粒子的影响。黑暗条件和适当高温有助于纳米银粒子的生成及其抑菌活性的增强。随着p H的增加,纳米银特征性吸收峰不断增强,但抑菌活性呈现先增强再减弱的趋势,p H 7~8范围内得到的纳米银粒子的抑菌活性较高。调节F.graminearum DO-1菌体浸泡液的p H为7,与终浓度为1 m M的Ag NO3溶液于80℃、黑暗条件下反应3 h,获得纳米银粒子,透射电镜分析显示制备的纳米银粒子为球形,直径范围2~50 nm。应用该内生真菌代谢产物制备具有抑菌功能的纳米银粒子,方法简单,条件温和,复合绿色生态的发展趋势。
[Abstract]:An endophytic fungus DO-1 was isolated from Dendrobium. The strain was identified as Fusarium graminearum. The metabolites secreted by Fusarium graminearum in deionized water reacted with silver nitrate. Silver nanoparticles with antimicrobial activity were produced by reducing silver ions. The characteristic plasma absorption peaks of silver nanoparticles in the range of 440 nm and the bacteriostatic activity of the products were used as indexes to study the illumination conditions. The effects of temperature and pH conditions on the preparation of silver nanoparticles were investigated. Dark conditions and appropriate high temperatures contributed to the formation of silver nanoparticles and the enhancement of their bacteriostatic activities. With the increase of pH, the characteristic absorption peaks of silver nanoparticles increased. However, the bacteriostatic activity of silver nanoparticles obtained in the range of pH 7O 8 was higher than that in the range of p H 7O 8. The pH value of F graminearum DO-1 soaking solution was 7, and that of Ag NO3 solution with the final concentration of 1 mm was 80 鈩，

本文编号：1565518