新型荧光碳量子点制备及应用研究
发布时间:2018-03-10 22:19
本文选题:碳量子点 切入点:蛋白质 出处:《辽宁大学》2017年硕士论文 论文类型:学位论文
【摘要】:近年来,碳量子点因其卓越的光学、化学、生物学特性,受到了科研人员极大地关注。相比其他的荧光纳米材料,如由重金属作为重要组成的高荧光性半导体量子点,碳量子点具有生物相容性更好,制备方法更简单、绿色等特点。本论文通过微波法处理两种不同的碳源,成功地制备了两种碳量子点,并通过多种分析手段对两种碳量子点进行了表征。与此同时,又对这两种碳量子点的性质进行了深入研究,得到了如下结论:(1)通过一步微波法处理变性蛋白质(dBSA)制备碳量子点,这是一种简单、绿色的制备高荧光碳量子点的方法。在这个体系中,用微波法制备碳量子点节约了大量的时间和能量,同时抑制副反应发生。通过此方法制备的变性蛋白质碳量子点(DPCDs)的荧光量子产率可达到13.3%。同时,DPCDs具有良好的上转换性质。此外,又对DPCDs的形成机理进行了研究,提出了DPCDs形成所需经历的四个阶段,包括:交联、芳构化、成核、增长。另外,制备的DPCDs还表现出了对Ag~+的选择性,可以用于检测Ag~+。当Ag~+浓度为0.1到10.0μM时,淬灭率和Ag~+浓度之间具有一个R2=0.991的拟合曲线。(2)通过绿色、简单的微波法处理离子液体(ILs,[PAVIm~+][Br-])和乙二胺混合物制备了碳量子点。离子液体基碳量子点(ILCDs)对Cr(VI)展现了良好的选择性和灵敏性。当Cr(VI)浓度在0.05 10.0μM范围内,ILCDs荧光强度和Cr(VI)浓度之间展现了良好的线性关系,线性方程为:(F0-F)/F0=0.0222×CCr(μM)~+0.0047,(R2=0.994),检出限为0.015μM(S/N=3)。而世界卫生组织(WHO)对饮用水中Cr(VI)含量规定为50.0μg L-1(约1.0μM),这个浓度刚好在ILCDs对Cr(VI)的检测范围内。在实际样品中对Cr(VI)检测,得到了令人满意的结果。此外,ILCDs也可以作为荧光探针检测溶液中pH和温度变化。
[Abstract]:In recent years, carbon quantum dots have attracted great attention due to their excellent optical, chemical and biological properties. Compared with other fluorescent nanomaterials, such as highly fluorescent semiconductor quantum dots composed of heavy metals, Carbon quantum dots have the advantages of better biocompatibility, simpler preparation method and green. In this paper, two kinds of carbon quantum dots were successfully prepared by microwave treatment of two different carbon sources. At the same time, the properties of the two kinds of carbon quantum dots were studied. The conclusion is as follows: 1) carbon quantum dots are prepared by one-step microwave treatment of denatured protein dBSAs. This is a simple, green method for preparing high fluorescent carbon quantum dots. In this system, microwave preparation of carbon quantum dots saves a lot of time and energy. The fluorescence quantum yield of denatured protein carbon quantum dots (DPCDs) obtained by this method can reach 13.3. At the same time, DPCDs have good up-conversion properties. In addition, the formation mechanism of DPCDs is also studied. Four stages of formation of DPCDs were proposed, including crosslinking, aromatization, nucleation and growth. In addition, the prepared DPCDs showed selectivity to Ag ~, and could be used to detect Ag ~-when Ag~ concentration was 0.1 to 10.0 渭 M. There is a fitting curve of R2O0.991 between quenching rate and Ag- concentration. Carbon quantum dots (QDs) were prepared by simple microwave treatment of ionic liquids (ILs), [PAVIm-] [Br-] and ethylenediamine mixtures. Ionic liquid-based carbon quantum dots (CQDs) showed good selectivity and sensitivity to CrVI. The fluorescence intensity of ILCDs was obtained in the range of 0.05 渭 M for CrVI and 10.0 渭 M for ethylene diamine. And CrVI concentrations show a good linear relationship, The linear equation is 0. 0222 脳 CCr1 (渭 M ~ 0. 0047 ~ 0. 007 ~ 0. 07), and the detection limit is 0. 015 渭 M ~ (-1). The World Health Organization (WHO) prescribes that the content of CrVI in drinking water is 50. 0 渭 g L ~ (-1). This concentration is within the detection range of ILCDs for CrVI. In actual samples, CrVI is detected. In addition, the ILCDs can also be used as a fluorescence probe to detect the pH and temperature changes in the solution.
【学位授予单位】:辽宁大学
【学位级别】:硕士
【学位授予年份】:2017
【分类号】:O657.3;TB383.1
【相似文献】
相关期刊论文 前10条
1 吴峰;蔡继业;;基于量子点的荧光共振能量转移的应用[J];化学与生物工程;2007年01期
2 季雷华;高素莲;张斌;;量子点的合成、毒理学及其应用[J];环境化学;2008年05期
3 王富;刘春艳;;发光碳量子点的合成及应用[J];影像科学与光化学;2011年04期
4 伊魁宇;王猛;邵明云;;量子点作为离子探针的分析应用[J];广州化工;2012年11期
5 罗慧;李曦;方婷婷;刘鹏;;量子点的毒性研究进展[J];材料导报;2013年19期
6 田瑞雪;武玲玲;赵清;胡胜亮;杨金龙;;碳量子点的氨基化及其对发光性能的影响[J];化工新型材料;2014年01期
7 ;“量子点”晶体将推动部分物理工艺的进步[J];光机电信息;2002年10期
8 徐万帮;汪勇先;许荣辉;尹端l,
本文编号:1595313
本文链接:https://www.wllwen.com/kejilunwen/huaxue/1595313.html
教材专著