低聚壳聚糖衍生物与BSA的作用研究

发布时间：2018-03-13 05:25

本文选题：低聚壳聚糖　切入点：衍生物　出处：《光谱学与光谱分析》2017年06期 　论文类型：期刊论文

【摘要】：研究了具有生物相容性的低聚壳聚糖衍生物与活性药物牛血清白蛋白(BSA)之间的作用。合成了聚合度为20的窄分子量分布低聚壳聚糖(NLCS_(20))的3种衍生物:N-马来酰化低聚壳聚糖(MNLCS)、N-羧甲基低聚壳聚糖(CNLCS)和N-琥珀酰化低聚壳聚糖(SNLCS)。通过紫外光谱(UV)、荧光光谱和等温滴定微量热仪(ITC)研究了NLCS_(20)衍生物与BSA之间的相互作用。结果表明,BSA的构象在NLCS_(20)衍生物溶液微环境中没有发生变化,活性也没有受到影响。NLCS_(20)衍生物与BSA的作用过程是放热的且是自发进行的,主要作用力是氢键和疏水作用。说明低聚壳聚糖衍生物能够作为潜在的亲水药物如活性医药蛋白或氨基酸的运输载体。
[Abstract]:The interaction between biocompatible chitosan derivatives and bovine serum albumin (BSA) was studied. The interaction between BSA derivatives and their derivatives was studied by UV spectra, fluorescence spectra and isothermal titration microcalorimeter. The interaction between the derivatives and BSA was studied by means of UV spectra, fluorescence spectra and isothermal titration microcalorimeter. The results show that the interaction of NLCSS-20) derivatives with BSA has been studied by means of UV spectra, fluorescence spectra and isothermal titration microcalorimeter (ITC-). The results show that the interaction of NLCSS-20) derivatives with BSA has been studied by UV spectra, fluorescence spectra and isothermal titration microcalorimeter. The conformation did not change in the microenvironment of the NLCSS-20) derivative solution, The interaction of the derivatives with BSA is exothermic and spontaneous. The main force is hydrogen bond and hydrophobic interaction, which indicates that oligomeric chitosan derivatives can be used as transport carriers of potential hydrophilic drugs such as active pharmaceutical proteins or amino acids.
【作者单位】：江苏大学化学化工学院;海南精细化工重点实验室;
【基金】：国家自然科学基金项目(21601068) 江苏省博士后科研资助计划项目(1401050C) 江苏省高校自然科学研究面上项目(14KJB150006) 江苏大学高级人才科研启动基金项目(14JDG054)资助
【分类号】：O636.1;TQ460.1

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