ICP-MS对牡丹皮中24种微量元素的形态及其溶出特性分析

发布时间：2018-03-14 20:38

本文选题：牡丹皮　切入点：形态分析　出处：《中国实验方剂学杂志》2017年03期 　论文类型：期刊论文

【摘要】：目的:对牡丹皮中Fe,Mn,Cu,Zn,Al,Pb,As,Cd等24种微量元素采用煎煮法进行提取。方法:使用0.45μm的微孔滤膜分离提取液中的悬浮态和可溶态,将可溶态通过大孔树脂(LSA-10),以去离水和甲醇洗脱,确定微量元素的无机态和有机态;模拟人体胃肠溶液,采用正辛醇-水系统分离微量元素的醇溶态和水溶态;通过ICP-MS技术(电感耦合等离子体质谱法),测定微量元素总量及各种形态。结果:方法的回收率为74.7%~118.2%,精密度RSD在1.3%~6.9%。24种微量元素的溶出率为2.0%~82.9%,可溶态在水中的比率为73.6%~99.5%,可溶态中80%以上微量元素以无机态和水溶态形式存在。结论:微量元素的形态分析及溶出特征为牡丹皮的临床应用提供理论依据。
[Abstract]:Objective: to extract 24 trace elements from peony peel, such as Fe, mn, Cu, Zn, Al, Pb, and CD, etc. Methods: suspension state and soluble state of the extract were separated by 0.45 渭 m microporous membrane, and the soluble state was separated through macroporous resin LSA-10 for water removal and methanol elution. The inorganic and organic states of trace elements were determined, and the alcohol-soluble and water-soluble states of trace elements were separated by using n-octanol-water system to simulate human gastrointestinal solution. ICP-MS technique (inductively coupled plasma mass spectrometry) was used to determine the total amount and various forms of trace elements. Results: the recovery rate of the method was 74.70.118.2%. The dissolution rate of 24 trace elements was 2.0 ~ 82.9%, and the ratio of soluble state in water was 2.0 ~ 82.9%. More than 80% trace elements in soluble form were found in inorganic and water-soluble forms. Conclusion: the morphological analysis and dissolution characteristics of trace elements provide theoretical basis for clinical application of peony peel.
【作者单位】：辽宁中医药大学药学院;江苏康缘药业股份有限公司;
【基金】：国家“重大新药创制”科技重大专项(2013ZX09402203)
【分类号】：R284.1;O657.63

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