通过荧光探针法测定西吡氯铵、苯扎溴铵和盐酸丁卡因

发布时间：2018-03-15 18:22

本文选题：葫芦[7]脲　切入点：盐酸巴马汀　出处：《山西师范大学》2017年硕士论文　论文类型：学位论文

【摘要】：荧光光谱法具有灵敏度高、选择性好及操作简便等特点而被广泛使用,然而某些有机药物分子本身没有荧光,所以不能直接通过荧光光谱法来测定。本文借助于第四代超分子主体葫芦[7]脲(CB[7])与天然异喹啉生物碱巴马汀(PAL)形成的荧光探针体系,研究了某些有机药物分子与荧光探针PAL在CB[7]空腔中的竞争反应。发现无荧光有机药物西吡氯铵、苯扎溴铵和盐酸丁卡因均能使CB[7]-PAL荧光探针体系产生强烈的猝灭作用,而且药物的浓度与荧光猝灭的速率呈良好的线性关系,据此分别建立了测定西吡氯铵、苯扎溴铵和盐酸丁卡因荧光光谱法。所建立的方法具有简单、快捷、选择性好、灵敏度高等优点,并将该方法应用到了药物制剂、血样及尿样的检测,检测结果取得令人满意的结果。另外本文采用荧光光谱、紫外光谱、1H NMR等技术探讨了这3种药物分子与探针在CB[7]空腔中的竞争反应机理,可以看出3种药物分子与CB[7]的包合常数均大于CB[7]-PAL的包合常数,因此在竞争反应中探针分子被药物分子挤出空腔而使探针荧光猝灭。最后通过密度泛函理论计算还建立了药物与超分子相互作用的分子模型。本文可认为四个部分:1、介绍了第4代超分子主体CB[n]的合成、分子结构、分子识别、分子组装和分子器件等领域的研究进展。简述了以CB[n]为主体的荧光增敏法研究和以CB[n]为主体的荧光猝灭法研究进展与现状。2、西吡氯铵(CPC)没有荧光,故至今没有荧光光谱法测定CPC的文献报道。CB[7]-PAL是一种可以发射强烈荧光的探针体系,随着CPC的加入荧光强度明显降低,且在一定范围内猝灭值与CPC的浓度成正比。通过一些先进技术探讨了其超分子作用机理,并计算了CB[7]-CPC超分子包合物的结合常数,据此为CPC建立了一种荧光光谱检测法。3、通过荧光光谱法研究了在酸性溶液中苯扎溴铵(BKB)与CB[7]的包合作用,BKB本身没有荧光,通过在CB[7]疏水空腔中与PAL的竞争反应,导致CB[7]-PAL体系荧光猝灭,猝灭值与BKB的浓度呈良好的线性关系,据此建立了一种测定BKB的灵敏度高、选择性好、重现性好的荧光光谱法。4、采用荧光光谱法,紫外光谱法和1H NMR研究了盐酸丁卡因(TCH)与CB[7]的相互作用,并通过密度泛函理论计算建立了TCH与CB[7]相互作用的分子模型。据此为TCH建立了一种以CB[7]为主体、PAL为探针的荧光光谱新方法,结果表明所建立的方法的灵敏度均高于文献报道的TCH光谱分析方法。且该方法可用于测定药物制剂和血样中TCH的含量。
[Abstract]:Fluorescence spectrometry is widely used because of its high sensitivity, good selectivity and simple operation. However, some organic drug molecules do not have fluorescence. Therefore, the fluorescence probe system formed by 4th generation supramolecular host gourd [7] Urea [7] CB [7] and natural isoquinoline alkaloid palmatine palmatine (PAL) was not directly determined by fluorescence spectrometry. The competitive reaction of some organic drug molecules with fluorescent probe PAL in CB [7] cavity was studied. It was found that the fluorescence probe system of CB [7] -pal could be quenched strongly by the non-fluorescent organic drug cepirium chloride, benzalkonium bromide and tetracaine hydrochloride. In addition, the concentration of the drug showed a good linear relationship with the rate of fluorescence quenching. Based on this method, a fluorescence spectrometric method for the determination of cepiramide, benzalkonium bromide and tetracaine hydrochloride was established. The method was simple, rapid and selective. The method has been applied to the detection of pharmaceutical preparations, blood samples and urine samples, and the results are satisfactory. In addition, fluorescence spectra are used in this paper. The competitive reaction mechanism of these three drug molecules and probes in CB [7] cavity was investigated by UV spectra 1H NMR and other techniques. It can be seen that the inclusion constants of the three drug molecules with CB [7] are larger than those of CB [7] -pal. Therefore, in the competitive reaction, the probe molecule is squeezed out of the cavity by the drug molecule to quench the fluorescence of the probe. Finally, the molecular model of the interaction between drug and supramolecular is established by density functional theory. In this paper, four parts can be considered. In this paper, the synthesis of the fourth generation supramolecular host CB [n] is introduced. The research progress in the fields of molecular structure, molecular recognition, molecular assembly and molecular devices. The progress and present situation of fluorescence sensitization method with CB [n] as the main body and fluorescence quenching method with CB [n] as the main body are briefly described. So far, there is no literature report on the determination of CPC by fluorescence spectroscopy. CB [7] -pal is a probe system which can emit strong fluorescence, and the fluorescence intensity decreases obviously with the addition of CPC. The quenching value is proportional to the concentration of CPC in a certain range. The mechanism of supramolecular interaction of CB [7] -CPC is discussed by some advanced techniques, and the binding constants of CB [7] -CPC supramolecular inclusion complexes are calculated. Based on this, a fluorescence spectrometric method for the detection of CPC was established. By means of fluorescence spectroscopy, the competitive reaction with PAL in the hydrophobic cavity of CB [7] was studied by means of fluorescence spectroscopy, which was used to study the inclusion of BKB with CB [7]. The fluorescence quenching of CB [7] -pal system resulted in a good linear relationship between the quenching value and the concentration of BKB. Based on this, a fluorescence spectrometric method with high sensitivity, good selectivity and good reproducibility for the determination of BKB was established. The interaction between tetracaine hydrochloride and CB [7] was studied by UV spectroscopy and 1H NMR. The molecular model of interaction between TCH and CB [7] was established by density functional theory, and a new fluorescence spectrum method with CB [7] as the probe was established for TCH. The results showed that the sensitivity of the established method was higher than that of the TCH spectrometric method reported in the literature, and the method could be used to determine the content of TCH in drug preparations and blood samples.
【学位授予单位】：山西师范大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：O657.3;R927

【参考文献】