基于萘酰亚胺和邻苯二甲酸酐的新型荧光探针的合成及性能研究

发布时间：2018-03-16 02:06

本文选题：荧光探针　切入点：1　出处：《郑州大学》2017年硕士论文　论文类型：学位论文

【摘要】：近年来,对于各种有机小分子、金属离子以及其他生物体内化合物的识别检测,是人们研究的重要领域之一。在各种检测方法中,荧光检测法由于其具有高的选择性、好的灵敏度以及实时检测等各种优点而受到广泛关注。在各类荧光探针的使用中,1,8-萘酰亚胺荧光团的应用研究非常活跃,其原因莫过于萘酰亚胺荧光团自身良好的光学性质:较高的光稳定性、较大的Storks位移和较强的荧光发射。本文基于1,8-萘酰亚胺设计了两个荧光探针即M_1、M_2,其中M_1可用于高选择性识别检测HS-,M_2可用于高灵敏度识别检测HS-和生物体中的氨基酸(GSH和Cys)。探针M_1、M_2均已通过细胞成像实验确定其具有实际应用潜力,为其后期在生物中的应用奠定了基础。邻苯二甲酸酐类荧光团具有合成步骤简单、成本低、水溶性很好等优点,本文基于该类荧光团设计并合成了探针M_3、M_4,分别用于比率检测pH和高选择性识别Cys。其中M_3已通过标准pH计的检测对比证实其检测结果的准确性,目前虽存在许多同时检测生物内硫醇的探针,但多数探针并不能将GSH和Cys进行区别检测,本文通过简单的步骤设计并合成的M_4则可以将对Cys与GSH、Hcy等生物硫醇的识别检测明显区分开,对Cys具有专一的选择性。针对该探针的实际应用,我们也做了相关试纸实验,得到了较为满意的结果。
[Abstract]:In recent years, the recognition and detection of various organic small molecules, metal ions and other biological compounds is one of the most important research fields. Good sensitivity, real-time detection and other advantages have attracted wide attention. In the use of various fluorescent probes, the application of 1-Naphthalimide fluorescence group is very active. The reason is that the naphthalimide fluorescence group has good optical properties: high light stability, Large Storks shifts and strong fluorescence emission. Two fluorescent probes, M1S / M _ 2, have been designed based on 1 ~ 8- naphthalimide, in which M _ s _ 1 can be used for highly selective identification and detection of HS-M _ (2), and can be used to detect HS- and amino groups in organisms with high sensitivity. Both GSH and Cyssyl probes have been confirmed by cell imaging experiments to have practical application potential. It lays a foundation for its later application in biology. Phthalic anhydride fluorescent group has the advantages of simple synthesis, low cost, good water solubility and so on. In this paper, we have designed and synthesized the probe M _ (3) M _ (4) based on this kind of fluorescence group, which has been used to detect pH ratio and identify Cyss with high selectivity, respectively. The accuracy of the detection results has been verified by the standard pH meter. Although there are many probes for simultaneous detection of mercaptan in organisms, most of the probes do not distinguish GSH from Cys. In this paper, we can distinguish the recognition and detection of biological mercaptan such as Cys and GSH Hcy by a simple step design and synthesis, and have a specific selectivity to Cys. In view of the practical application of this probe, we have also done the relevant test paper experiment. Satisfactory results have been obtained.
【学位授予单位】：郑州大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：O657.3

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