HPLC指纹图谱结合化学计量学评价不同产地广东紫珠药材的质量

发布时间：2018-03-18 05:16

本文选题：广东紫珠　切入点：HPLC　出处：《中草药》2017年01期 　论文类型：期刊论文

【摘要】：目的建立不同产地广东紫珠Callicarpa kwangtungensis的HPLC指纹图谱,用于广东紫珠药材质量的评价。方法采用中药色谱指纹图谱相似度评价系统(2004A版)对5省15批不同产地的广东紫珠药材的HPLC指纹图谱进行相似度评价,并使用SPSS 19.0软件进行聚类分析和主成分分析。结果选取了12个色谱峰作为指纹图谱共有峰,15批样品的相似度计算结果均大于0.900,说明各产地的药材有较好的一致性;通过聚类分析可将15批样品聚为4类;主成分分析用3个主成分对广东紫珠药材进行综合评价,综合得分结果显示,湖南会同、广西桂林、江西武宁、江西南昌、江西萍乡、江西宜春的广东紫珠药材在所有样品中的综合得分位于前6名。结论本实验建立的广东紫珠质量评价方法操作简便,重复性好,结果可靠,可以用于广东紫珠药材的质量控制和评价。
[Abstract]:Objective to establish HPLC fingerprint of Callicarpa kwangtungensis from different habitats. Methods the HPLC fingerprints of 15 groups of different producing areas in 5 provinces were evaluated by using the similarity evaluation system of chromatographic fingerprint of traditional Chinese medicine (2004A), which was used to evaluate the quality of purple pearl medicinal materials in Guangdong province. SPSS 19.0 software was used for cluster analysis and principal component analysis. Results 12 chromatographic peaks were selected as the common peaks of the fingerprint. The results of similarity calculation of 15 samples were all greater than 0.900, which indicated that there was good consistency among the samples from different producing areas. Through cluster analysis, 15 batches of samples can be grouped into 4 groups, and three principal components were used to evaluate the medicinal materials of purple pearl in Guangdong province. The comprehensive score showed that Hunan, Guilin, Wuning, Nanchang, Pingxiang, Jiangxi, The comprehensive scores of all samples were in the top 6. Conclusion the quality evaluation method established in this experiment is simple, reproducible and reliable. It can be used for quality control and evaluation of purple pearl medicinal materials in Guangdong.
【作者单位】：江西省林业科学院;
【基金】：国家青年科学基金项目(81102799)
【分类号】：R284.1;O657.72

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