新型三氰基二氢呋喃喹诺酮类荧光探针在药物分析中的研究

发布时间：2018-03-18 07:18

本文选题：新型三氰基二氢呋喃　切入点：喹诺酮　出处：《佳木斯大学》2017年硕士论文　论文类型：学位论文

【摘要】：目的通过合成新型三氰基二氢呋喃(DCDHF-2-V)电子受体,设计了(DCDHF-2-V)—喹诺酮类荧光探针。创建了一种检测喹诺酮类药物含量的新方法,并研究了其最优的实验条件,同时探讨了其增敏原理。方法首先设计、合成了一种新型电子受体-新型三氰基二氢呋喃,并通过一系列的方法表征了此物质结构。利用荧光分光光度分析法,首次建立了新型三氰基二氢呋喃-喹诺酮类荧光探针,并用于体外喹诺酮类药物含量的检测。以新型三氰基二氢呋喃为电子受体,配合了新型三氰基二氢呋喃-环丙沙星、新型三氰基二氢呋喃-诺氟沙星、新型三氰基二氢呋喃-氧氟沙星、新型三氰基二氢呋喃-加替沙星多种荷移络合物,成立了喹诺酮类药物含量测定的新方法。结果通过一定的反应条件,合成了一种新型电子受体-新型三氰基二氢呋喃,并通过一系列的化学分析和表征确定了其结构。在适宜的反应pH、比例、时间和温度下,新型三氰基二氢呋喃与喹诺酮类药物能够通过荷移反应形成稳定的络合物,并探讨了络合物形成的机理。此法用于体外检测喹诺酮类药物的含量,具有高灵敏度、高选择性、操作简便等优点。结论合成了新型电子受体-新型三氰基二氢呋喃,构建了新型三氰基二氢呋喃喹诺酮类药物荧光探针,确立了新型三氰基二氢呋喃与喹诺酮类药物络合最佳条件,开辟了新型喹诺酮类药物荧光探针的新思路,并且此种荧光探针能够用于体外测定喹诺酮类药物的含量,其结果准确有效。
[Abstract]:Objective through the synthesis of new three - two hydrogen furan (DCDHF-2-V) electron acceptor, design (DCDHF-2-V) - quinolone fluorescent probe. A new method for creating a detection of quinolone drug content, and to study the optimal experimental conditions, and discusses its sensitivity principle. Firstly the design and synthesis of a novel three - two new electron acceptor hydrogen furan, and characterized by a series of methods of the material structure. Using fluorescence spectrophotometric method was established for the first time, the new three - two hydrogen furan - quinolone fluorescent probe, and detected with in vitro quinolone content. With a new three cyano two hydrogen furan as electron acceptor with the new three, ciprofloxacin two hydrogen furan - cyano, new three cyano two hydrogen furan - norfloxacin, new three cyano two hydrogen furan - floxacin, new three cyano two hydrogen furan - more than gatifloxacin A charge transfer complex, set up a new method for determination of quinolones. The reaction conditions of the synthesis of a novel electron acceptor model three cyano two hydrogen furan, and their structures were determined by chemical analysis and characterization of a series of pH in the reaction. The proportion, suitable, time and temperature a new three - two hydrogen furan and quinolones by charge transfer reaction to form a stable complex, and to explore the mechanism of complex formation. This method was used for the content of in vitro detection of quinolones, with high sensitivity, high selectivity, easy operation etc.. The new electronic receptor model three - two hydrogen furan synthesis conclusion, construction a new three - two hydrogen furan quinolone fluorescent probe, established the optimum conditions of a new type of three - two hydrogen furan complexed with quinolones, opened up a new quinolone A new idea of the drug fluorescent probe, and the fluorescence probe can be used for the determination of quinolones in vitro, and the results are accurate and effective.

【学位授予单位】：佳木斯大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：O657.3;TQ460.72

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