新型比率电化学传感器的构建及其生化分析应用研究

发布时间：2018-03-18 12:54

本文选题：电化学传感器　切入点：比率　出处：《青岛大学》2017年硕士论文　论文类型：学位论文

【摘要】：生物体内一些重要分子,如:盐酸阿霉素(DOX)等抗肿瘤药物,多巴胺(DA)等疾病标志物的检测对人体健康以及临床生化分析意义重大。荧光法,比色法,电泳法,色谱法等常见的分析方法面临耗费高,样品制备复杂等问题。电化学传感器由于灵敏度高,响应快,简便,成本低,体内检测等特点,受到广泛关注。传统的单信号电化学传感器对实验室检测条件的严重依赖的技术缺陷,限制了它的发展应用。比率电化学传感器引入了内在校准机制,可有效降低传感体系内的背景电信号,进而提高电化学传感的准确性,重现性和灵敏性。基于此,本课题基于电化学比率技术构建了新型的比率电化学生物传感器对DOX、DA进行检测,具体研究如下:一、设计了一种直接基于裸玻碳电极检测常规电解液中的目标物的新型双比率电化学DOX传感器。本研究选取具有不同氧化峰电位的三种物质,提供内部参比信号的二茂铁(Fc,0.15 V)和亚甲基蓝(MB,-0.26 V),以及作为分析物的DOX(-0.62 V)后,制备出三者的混合电解液体系。通过直接使用裸玻碳电极作为双信号传感平台,测量含有固定浓度Fc、MB和不同浓度DOX的三种组分的各电解质溶液的方波伏安曲线。实验结果表明,该传感体系具有类似甚至优于其他修饰电极体系的宽线性范围和低检测限。此外,该双比率电化学传感器具有良好的选择性和稳定性,可用于实际血清和唾液样品中DOX的检测。该双比率电化学传感器简易、新颖,且具备一定的普遍性,可发展出一系列的新型双比率电化学传感体系。二、构建了一种基于金纳米离子/电化学还原石墨烯-耐尔蓝(AuNPs/ERGO-NB)纳米复合材料修饰玻碳电极的新型免标记比率电化学DA适体传感器。基于AuNPs/ERGO-NB纳米复合材料优异的导电性,适体对分析物的特异性,以双信号电流比值(IDA/INB)作为信号输出,该比率电化学适体传感器表现出长时间稳定性和优异的重现性,可以对DA实现高灵敏性,高选择性,和准确性地检测。此外,该传感器具备实际样品检测能力,可应用于实际血清样品中的DA的分析。更重要的是,该分析方法可以通过结合特定受体和靶目标物,容易地扩展到用于检测其它电活性物质的新型电化学传感器的设计。
[Abstract]:The detection of some important molecules, such as doxorubicin hydrochloride and dopamine (DAA), is of great significance to human health and clinical biochemical analysis. Common analytical methods, such as chromatography, face high cost and complex sample preparation. Electrochemical sensors are characterized by high sensitivity, fast response, simplicity, low cost and in vivo detection. The traditional single signal electrochemical sensor, which is heavily dependent on the laboratory detection conditions, has limited its development and application. The ratio electrochemical sensor has introduced the inherent calibration mechanism. It can effectively reduce the background signal in the sensing system and improve the accuracy, reproducibility and sensitivity of the electrochemical sensor. Based on the electrochemical ratio technology, a novel ratio electrochemical biosensor was constructed to detect DOXDA. The main results are as follows: 1. A novel dual ratio electrochemical DOX sensor based on bare glassy carbon electrode was designed to detect the target in conventional electrolyte. In this study, three kinds of substances with different oxidation peak potentials were selected. A mixture electrolyte system of ferrocene (Fc0. 15 V), methylene blue (MB-0.26 V) and DOX(-0.62 V (as an analytical material) was prepared by direct use of bare glassy carbon electrode as a dual signal sensing platform. The square wave voltammetry curves of three electrolyte solutions with fixed concentrations of Fcmb and different concentrations of DOX were measured. The sensor system has a wide linear range and a low detection limit similar to or even superior to that of other modified electrode systems. In addition, the dual ratio electrochemical sensor has good selectivity and stability. It can be used for the detection of DOX in serum and saliva samples. The dual ratio electrochemical sensor is simple, novel and universal, and a series of novel dual ratio electrochemical sensing systems can be developed. A novel labeling free ratio electrochemical DA aptamer sensor based on au nano ion / electrochemical reduction graphene / Nel blue (AuNPs / ERGO-NB) nanocomposite modified glassy carbon electrode was constructed. Based on the excellent conductivity of AuNPs/ERGO-NB nanocomposites, a novel electrochemical aptamer sensor was developed. The specificity of the aptamer to the analyte. The ratio of double signal current to IDA / INB is used as the signal output. The electrochemical aptamer sensor has a long time stability and excellent reproducibility, which can achieve high sensitivity and selectivity to DA. In addition, the sensor has the ability to detect actual samples and can be applied to the analysis of DA in actual serum samples. Easy to extend to the design of new electrochemical sensors for the detection of other electrically active substances.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：TP212;O657.1

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