测定美白类化妆品中氢醌和苯酚的高效液相色谱法改进研究

发布时间：2018-03-18 13:50

  本文选题：氢醌　切入点：苯酚　出处：《药物分析杂志》2017年03期 　论文类型：期刊论文

【摘要】：目的:对高效液相色谱法测定美白类化妆品中氢醌、苯酚的色谱条件进行优化。方法:以系统适用性为指标,比较了色谱柱类型、缓冲盐种类及流动相p H对氢醌、苯酚和化妆品样品分离效果的影响。对优化后色谱条件进行了方法学考察,并在此条件下对抽检的化妆品样品进行测定。结果:最优色谱条件:采用C_(18)(4.6 mm×250 mm,5μm)色谱柱,以10 mmol·L~(-1)磷酸二氢钾水溶液(磷酸调p H 3.5)-甲醇为流动相进行梯度洗脱,流速1mL·min~(-1),柱温25℃,检测波长280 nm,进样量5μL。在最优色谱条件下,氢醌质量浓度在0.4~40μg·mL~(-1)范围内,苯酚质量浓度在0.7~70μg·mL~(-1)范围内线性关系良好,相关系数分别为0.999 8和0.999 9,氢醌、苯酚检测限分别为0.000 5和0.001μg,定量限分别为0.002和0.003 5μg,精密度试验的RSD分别为0.4%~1.0%和0.3%~1.8%,回收率分别为98.4%~99.5%和100.4%~102.2%。对市售的美白祛斑类化妆品200批进行测定,在本文的样品处理方法下氢醌和苯酚的检出限分别为1和2μg·g~(-1),定量限分别为4和7μg·g~(-1),杂质峰和目标峰能够实现基线分离、峰形对称。结论:本法测定美白祛斑类化妆品中氢醌、苯酚含量的专属性好,可以为化妆品检验标准的改进提供参考。
[Abstract]:Objective: to optimize the chromatographic conditions for the determination of hydroquinone and phenol in whitening cosmetics by high performance liquid chromatography. The effect of phenol and cosmetics on the separation of samples was investigated. The optimized chromatographic conditions were investigated, and the samples of cosmetics were determined under these conditions. Results: the optimum chromatographic conditions were as follows: Che 184.6mm 脳 250mm 脳 5 渭 m) column. 10 mmol 路L ~ (-1) potassium dihydrogen phosphate solution was used as mobile phase, flow rate was 1 mL 路min ~ (-1) ~ (-1), column temperature was 25 鈩，

本文编号：1629848