山楂体外降三酰甘油主要活性组分谱效关系研究

发布时间：2018-03-19 09:48

本文选题：山楂　切入点：黄酮　出处：《中草药》2017年02期 　论文类型：期刊论文

【摘要】：目的探讨山楂降三酰甘油(TG)主要活性成分UPLC指纹图谱与药效之间的相关性。方法利用TG体外模拟消化模型,筛选出山楂降TG主要活性组分。采用UPLC法建立12批不同产地山楂降TG主要活性组分指纹图谱,并获取相应的药效数据,运用偏最小二乘法,进行相关性分析。结果山楂中富含黄酮的组分能够明显地降低TG水平。经相关性分析,在该组分指纹图谱所含的11个共有峰中,1(B型原花青素二聚体)、3(原花青素C1)、4(B型原花青素二聚体)、5(芦丁)、6(金丝桃苷)、7(异槲皮苷)、8(B型原花青素二聚体)号色谱峰所对应的化合物对降TG呈正相关作用,其余包括2(表儿茶素)、10(槲皮素)、11(5-O-咖啡奎尼丁酯)号色谱峰在内的各峰则呈负相关。结论山楂黄酮类成分对降低TG水平存在不同程度的影响。
[Abstract]:Objective to study the correlation between UPLC fingerprint and pharmacodynamics of the main active component of Triglyceride Triglyceride in Hawthorn. Methods the digestion model was simulated by TG in vitro. The main active components of reducing TG in Hawthorn were screened out. The fingerprint of 12 groups of active components of reducing TG in Hawthorn from different producing areas were established by UPLC method, and the corresponding effective data were obtained, and the partial least square method was used. Results the flavonoid rich components in Hawthorn could significantly reduce TG level. Among the 11 common peaks in the fingerprint chromatogram of this component, the peak number of the chromatographic peak of 1 / B type procyanidin dimer (C _ (1) C _ (1)) ~ (4) ~ (4) ~ (B) procyanidin dimer (Rutin) is corresponding to the peak number of the chromatographic peak of "Rutin" ~ (6) (Hypericrin (isoquercetin) ~ (8) B type procyanidin dimer). There was a positive correlation between the compound and TG, The other peaks, including the chromatographic peak of 2 (epigallocatechin) (quercetin (quercetin) 115-O- coffee quinyl ester), were negatively correlated. Conclusion Hawthorn flavonoids have different effects on the decrease of TG level.
【作者单位】：江西中医药大学现代中药制剂教育部重点实验室;中国药科大学;
【基金】：国家自然科学基金资助项目(81260638) 江西省自然科学基金项目(20161BAB205220)
【分类号】：R284;O657.7

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