滤纸SERS基底的制备及其用于精浆检测的研究

发布时间：2018-03-21 10:05

本文选题：表面增强拉曼散射　切入点：滤纸　出处：《福建师范大学》2016年硕士论文　论文类型：学位论文

【摘要】：表面增强拉曼光谱技术作为一种常用的分析方法,具有检测灵敏度高、测试速度快和非侵入性等优点,已经在生物医学、环境、食品安全等领域中获得了广泛关注。光谱测量时使用的增强基底既是检测物的承载基底,同时又对检测信号的增强有着重要作用。本文以基于滤纸制备的表面增强拉曼散射(SERS)基底为研究对象,通过对其增强性能的研究,获得一种可实现简单快速制备且灵敏度高、均匀性好、稳定性强的滤纸SERS基底的方法,并将这种滤纸基底用于精浆SERS光谱的检测中。主要的研究工作如下：1.使用浸泡法制备经过不同处理的滤纸SERS基底,并对其增强性能进行研究。首先获得了经过不同处理(不同NaCl浓度、不同胶体浸泡时间)制得的滤纸基底的扫描电镜图,对滤纸SERS基底表面银纳米粒子的分布情况进行对比分析,随后以R6G为探针分子对制得的滤纸基底的灵敏性、均一性和时间稳定性进行了研究。结果表明,NaCl浓度和浸泡胶体时间都会对银纳米粒子在滤纸基底上的分布产生重要影响,经过浓度为20mM的NaCl溶液预处理,并在浓缩银溶胶浸泡1-2h后制得的滤纸SERS基底,其增强因子就可达到2 107,对R6G溶液的检出限为10-7M,显示出很高的灵敏度。此外,通过对滤纸基底均一性和稳定性的研究表明,与未经过任何预处理的滤纸制备的SERS基底相比,经过预处理制备的滤纸基底也可以呈现出比较好的均一性和时间稳定性。2.将制得的滤纸SERS基底用于精浆SERS光谱的检测,通过分析精浆SERS光谱特征峰谱峰强度及强度变化的相对标准差,比较不同处理条件制备的滤纸基底用于实际样品检测时的增强性能和测试效果差异,对滤纸SERS基底的可靠性和均一性作出评价。结果表明,使用浓度为20 mM的NaCl溶液预处理并在银溶胶中浸泡1-2 h后即可获得测试效果很好的滤纸基底。本论文的初步研究成果表明：采用浸泡法可以简便地制备出可靠的滤纸SERS基底,而将滤纸使用NaCl溶液进行预处理可大大缩短基底的制备时间,这种灵敏性高、均匀性好、稳定性强的滤纸SERS基底将很有希望应用于犯罪现场体液痕迹鉴定、环境污染物检测、食品农药残留等实际检测中。
[Abstract]:As a common analytical method, surface-enhanced Raman spectroscopy has been widely used in biomedicine, environment, and has the advantages of high detection sensitivity, fast testing speed and non-invasive. The enhanced substrate used in spectral measurement is the carrier substrate for the detection. At the same time, it plays an important role in the enhancement of detection signal. In this paper, the surface enhanced Raman scattering (SERS) substrate based on filter paper is taken as the research object. Through the study of its enhancement performance, a simple and rapid preparation with high sensitivity is obtained. The method of SERS substrate with good uniformity and strong stability is used in the detection of SERS spectrum of seminal pulp. The main research work is as follows: 1. The filter paper SERS substrate with different treatment is prepared by immersion method. At first, the scanning electron microscope (SEM) of filter paper substrate prepared by different treatment (different NaCl concentration, different colloid immersion time) was obtained. The distribution of silver nanoparticles on the surface of filter paper SERS substrate was compared and analyzed, and the sensitivity of the prepared filter paper substrate was obtained by using R6G as probe molecule. The homogeneity and time stability were studied. The results showed that the distribution of silver nanoparticles on the filter paper substrate was significantly affected by the concentration of NaCl and the time of soaking the colloid. The silver nanoparticles were pretreated with 20 mm NaCl solution. The enhancement factor of filter paper SERS prepared by immersion of concentrated silver sol for 1-2 hours can reach 2107, and the detection limit of R6G solution is 10-7M. in addition, the uniformity and stability of filter paper substrate are studied. Compared with the SERS substrate prepared without any pretreatment, the filter paper substrate prepared by pretreatment can also show good uniformity and time stability. 2. The prepared filter paper SERS substrate can be used to detect the SERS spectrum of seminal plasma. By analyzing the relative standard deviation of the peak intensity and intensity change of seminal plasma SERS spectrum characteristic peak, the enhancement performance and testing effect of filter paper substrate prepared under different treatment conditions for actual sample detection were compared. The reliability and uniformity of filter paper SERS substrate were evaluated. The filter paper substrate was obtained by pretreatment with 20 mm NaCl solution and immersion in silver sol for 1-2 hours. The preliminary research results of this paper show that the reliable filter paper SERS substrate can be easily prepared by immersion method. The pretreatment of filter paper with NaCl solution can greatly shorten the preparation time of the substrate. The filter paper SERS substrate with high sensitivity, good uniformity and strong stability will hopefully be used in the identification of body fluid traces at the crime scene and the detection of environmental pollutants. Food pesticide residues and other practical detection.
【学位授予单位】：福建师范大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：O657.37

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