表面活性剂辅助分散液相微萃取-气相色谱法分析蔬菜水果中有机磷类农药残留

发布时间：2018-03-28 18:56

本文选题：表面活性剂　切入点：分散液相微萃取　出处：《分析科学学报》2017年01期

【摘要】：建立了测定蔬菜水果中有机磷类农药残留的表面活性剂辅助分散液相微萃取-气相色谱法。优化的萃取条件为:在5.0 mL样品溶液中加入50.0μL三氯甲烷和0.5mL 0.04 mmol·L-1的N-十二烷基-β-氨基丙酸盐表面分散剂,分散混匀后,以5 000r·min-1离心5min,吸取三氯甲烷相直接进样分析。在最优化条件下,8种有机磷类农药在0.02~0.50μg·mL-1范围内具有良好的线性关系,相关系数在0.9948~0.9985之间;检出限为0.0056~0.0110μg·mL-1。有机磷类农药的富集倍数高达264~537倍。方法应用于蔬菜水果中的有机磷类农药分析,加标回收率在82.4%~114.5%之间;相对标准偏差(RSD)在4.9%~13.7%之间。该方法具有操作简单、富集效率高和灵敏度高等特点,可满足蔬菜水果中有机磷类农药残留的检测。
[Abstract]:A method for determination of organophosphorus pesticide residues in vegetables and fruits by surfactant assisted dispersive liquid phase microextraction gas chromatography was established. The optimum extraction conditions were as follows: 50.0 渭 L trichloromethane and 0.5mL 0.04 mmol L-1 N- in 5.0 mL sample solution. Dodecyl 尾 -aminopropionate surface dispersant, After dispersing and mixing, centrifuging with 5 000r min-1 for 5 mins, direct injection analysis of trichloromethane phase was carried out. Under the optimum conditions, 8 organophosphorus pesticides had a good linear relationship in the range of 0. 02 渭 g mL-1, the correlation coefficient was between 0. 9948 and 0. 9985; The detection limit was 0.0056 ~ 0.0110 渭 g mL ~ (-1). The enrichment ratio of organophosphorus pesticides was 264N ~ (537) times. The method was applied to the analysis of organophosphorus pesticides in fruits and vegetables. The recovery rate was between 82.44% and 114.5%, and the relative standard deviation (RSD) was between 4.9% and 13.7%. The enrichment efficiency and sensitivity are high, which can be used for the determination of organophosphorus pesticide residues in vegetables and fruits.
【作者单位】：黑龙江八一农垦大学农学院;黑龙江八一农垦大学测试中心;农业部农产加工品质量监督检验测试中心;
【基金】：黑龙江八一农垦大学博士启动资金(No.XDB2015-24)
【分类号】：TS255.7;O657.71

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