基于铂金纳米复合材料电化学免疫传感器的构建及其性能研究

发布时间：2018-03-30 01:29

本文选题：贵金属纳米复合物材料　切入点：电化学免疫传感器　出处：《太原理工大学》2017年硕士论文

【摘要】：癌症,仍是人类主要的死亡原因。研究发现早期诊断和治疗是降低恶性肿瘤死亡率的有效途径,而肿瘤标志物为肿瘤疾病的早期诊断提供了重要的临床应用价值。本文主要制备了几种具有特殊性质的纳米复合材料用来构建良好性能的电化学免疫传感器,提高单个肿瘤标志物的灵敏度,开发多个肿瘤标志物的同时检测。主要研究内容和结果如下:提出并构建了基于三重信号放大策略的电化学免疫传感器,并将其用于AFP的检测。首先对二茂铁二羧酸进行光照处理合成具有电化学活性的无限配位聚合物纳米球,然后用铂纳米颗粒(Pt NP_S)和血红素/G-四联体(DNA zyme)对聚合物纳米球进行功能化修饰,再通过对H2O2的高效催化进一步增强电化学信号,最后将该复合物作为信号标记物修饰二抗构建夹心型电化学免疫传感器。研究结果表明,在最佳条件下采用差分脉冲伏安法测得的DPV峰随AFP浓度的增加而增加,线性检测范围为0.1 pg/mL~100 ng/mL,最低检测限为0.086 pg/mL。该电化学传感器制备方法简便、灵敏度高,对AFP具有良好的选择性。在上述研究的基础上,进一步构建了同时可对AFP和CEA进行特异性检测的电化学免疫传感器。先以Au@Pt壳核纳米颗粒为单元,分别制备了1,1’—二茂铁二甲酸/金@铂纳米复合物(Fc/Au@Pt)和硫堇/金@铂纳米复合物(Thi/Au@Pt)两种修饰二抗的信号标记物,然后将其分别标记AFP和CEA抗体进而构建夹心型免疫传感器,利用二茂铁(Fc)和硫堇(Thi)的峰电位不同且互不干扰的特性,实现了对AFP和CEA的同时检测。在最优条件下该传感器对AFP和CEA的检测限分别为2.6pg/mL和3.2 pg/mL。该电化学免疫传感器呈现出良好的选择性、稳定性和重现性。
[Abstract]:Cancer is still the leading cause of death in humans. Early diagnosis and treatment have been found to be an effective way to reduce the mortality rate of malignant tumors. Tumor markers provide important clinical value for the early diagnosis of tumor diseases. In this paper, several kinds of nano-composites with special properties were prepared to construct electrochemical immunosensors with good performance. The main contents and results are as follows: an electrochemical immunosensor based on triple signal amplification strategy is proposed and constructed. Firstly, ferrocene dicarboxylic acid was treated with light to synthesize infinite coordination polymer nanospheres with electrochemical activity. Then the polymer nanospheres were functionalized with Pt NPS and heme / G- quad DNA zyme, and the electrochemical signals were further enhanced by the efficient catalysis of H2O2. Finally, the complex was used as a signal marker to modify the second antibody to construct a sandwich electrochemical immunosensor. The results showed that the DPV peak measured by differential pulse voltammetry increased with the increase of AFP concentration. The linear detection range is 0.1 pg/mL~100 / mL and the lowest detection limit is 0.086 PG / mL. The electrochemical sensor is simple, sensitive and selective to AFP. An electrochemical immunosensor, which can detect both AFP and CEA, was further constructed. Firstly, the Au@Pt shell core nanoparticles were taken as the unit. Two kinds of signal markers were prepared for modifying the second antibody, I. e., 1H _ 1C ~ (-1) -ferrocene dicarboxylic acid / gold @ platinum nanocomposite (FC / Aufuture Pt) and thionine / gold @ platinum nanocomplex (Thir / Aur Pt), respectively, and then labeled with AFP and CEA antibodies respectively to construct a sandwich immunosensor. Using the characteristics of different peak potentials of ferrocene (Fc) and thionine (Thir), The detection limits of AFP and CEA are 2.6pg/mL and 3.2pg / mL, respectively. The electrochemical immunosensor has good selectivity, stability and reproducibility.
【学位授予单位】：太原理工大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：O657.1

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