温敏性乙二醇壳聚糖水凝胶的制备及药物缓释性能

发布时间：2018-04-01 02:17

  本文选题：乙二醇壳聚糖　切入点：乙酰化乙二醇壳聚糖　出处：《高等学校化学学报》2016年12期

【摘要】：以乙二醇壳聚糖为原料,乙酸酐为酰化剂,通过N-乙酰化反应,制得了新型温敏性高分子乙酰化乙二醇壳聚糖.通过核磁共振氢谱(1H NMR)、傅里叶变换红外光谱(FTIR)及试管倒置法对乙酰化乙二醇壳聚糖的结构及温敏性进行了表征,通过扫描电子显微镜(SEM)和紫外-可见分光光度计(UV-Vis)对水凝胶的微观形貌和体外药物释放性能进行了研究.结果表明,随着反应时间和乙酸酐与乙二醇壳聚糖氨基摩尔比的增加,产物的乙酰度逐渐增加;乙酰化乙二醇壳聚糖溶液具有热可逆温敏性溶胶-凝胶转变行为,可以通过控制乙酰化乙二醇壳聚糖的乙酰度和溶液浓度,使溶胶-凝胶转变温度处于室温至体温(25~37℃)之间;乙酰化乙二醇壳聚糖水凝胶具有"高度孔隙化且孔隙之间相互连通"的结构特点,通过控制乙酰度和溶液浓度,可使其孔径大小处于1~40μm范围内;乙酰化乙二醇壳聚糖水凝胶的乙酰度为89.90%时,质量分数为5%~7%的水凝胶对抗癌药物吉西他滨具有缓释作用,载药凝胶的释药时间可达3~5 d.乙酰化乙二醇壳聚糖有望在药物释放及组织工程等领域得到广泛应用.
[Abstract]:Using ethylene glycol chitosan as raw material and acetic anhydride as acylation agent, N-acetylation reaction was carried out. A novel thermo-sensitive macromolecule, acetylated ethylene glycol chitosan, was prepared. The structure and temperature sensitivity of acetylated ethylene glycol chitosan were characterized by 1H NMR, FTIR and tube inversion. The micromorphology of hydrogels and drug release properties in vitro were studied by scanning electron microscopy (SEM) and UV-Vis-UV spectrophotometer. The results showed that with the increase of reaction time and the molar ratio of acetic anhydride to ethylene glycol chitosan amino groups, The acetyl degree of the product increased gradually, and the acetylated ethylene glycol chitosan solution had the thermo-reversible sol-gel transition behavior, which could be controlled by controlling the acetyl degree and the solution concentration of acetylated ethylene glycol chitosan. The sol-gel transition temperature was between room temperature and temperature of 2537 鈩，

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