拉曼光谱在多组分定量分析中方法学验证的探讨

发布时间：2018-04-03 02:37

本文选题：方法学验证　切入点：拉曼光谱法　出处：《光谱学与光谱分析》2017年01期

【摘要】：拉曼光谱在中国药典2010版第一次被收录于"附录XIX L拉曼光谱法指导原则"中,但该原则对拉曼多组分定量分析的方法学验证未作论述,对多组分复杂体系中采用拉曼数学模型定量分析时的方法学验证进行独创性的探索和研究,分别从仪器性能测试、线性、定量限、准确度等方面进行方法学验证研究;另外由于拉曼光谱定量数学模型样品为复杂成分样品,无须像传统方法提供空白样品和进行破坏实验等专属性实验验证。通过研究探讨,提出了拉曼多组分定量分析的方法学验证内容,为方法学验证的完善和标准的制定提供了基础和依据。
[Abstract]:Raman spectroscopy was first included in the guidelines of XIX L Raman Spectrometry in Chinese Pharmacopoeia 2010. However, this principle did not discuss the methodological verification of quantitative analysis of Raman multicomponent.Original exploration and research on the methodology verification of quantitative analysis by Raman mathematical model in multicomponent complex system are carried out respectively from the aspects of instrument performance test linearity quantitative limit accuracy and so on.In addition, because the sample of quantitative mathematical model of Raman spectrum is a sample of complex components, it is not necessary to provide blank samples as traditional methods and to carry out specific experiments such as destruction experiments.The content of methodology verification of Raman multicomponent quantitative analysis is put forward, which provides the basis and basis for the perfection of methodology verification and the formulation of standards.
【作者单位】：新乡医学院药学院;四川大学华西药学院;新乡医学院三全学院药学院;成都中医药大学药学院;
【基金】：国家自然科学基金面上项目(81373969) 河南省高等学校重点科研项目(17A360026) 新乡医学院博士科研启动基金项目(505095)
【分类号】：O657.37

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