平贝母和川贝母总生物碱含量及其镇咳、抗炎作用比较研究

发布时间：2018-04-04 07:24

本文选题：平贝母　切入点：川贝母　出处：《食品工业科技》2017年15期

【摘要】：目的:比较平贝母与川贝母新品种——太白贝母、旧品种——松贝、青贝、炉贝总生物碱含量及镇咳、抗炎药理作用,探讨平贝母作为川贝母新来源的可行性。方法:采用回流法提取各品种总生物碱,紫外分光光度法测定其含量;比较不同品种贝母总生物碱对氨水刺激法致小鼠咳嗽模型和二甲苯致耳廓肿胀模型的药理作用;对各品种总生物碱咳嗽抑制率和炎症抑制率进行聚类分析。结果:各贝母提取物总生物碱含量从高到低依次为太白贝母(野生)、平贝母、青贝、松贝、炉贝、太白贝母(栽培)。平贝母、松贝、青贝、炉贝、太白贝母(栽培)总生物碱可明显减少氨水刺激法致小鼠咳嗽模型咳嗽次数,其中老品种较平贝母、新品种减少咳嗽次数优;松贝、青贝总生物碱可明显延长咳嗽潜伏期。平贝母、松贝总生物碱可明显减轻二甲苯致小鼠耳廓肿胀模型肿胀度;平贝母较松贝减轻抗炎效果更佳。聚类分析结果显示松贝、青贝、炉贝与太白贝母(栽培)、太白贝母(野生)聚集,与平贝母分离,表明平贝母与川贝母各品种总生物碱镇咳抗炎药效有一定差异。结论:平贝母总生物碱组成和抗炎、镇咳作用与川贝母有一定差异;但平贝母亦可作为川贝母候选新来源品种进一步研究。
[Abstract]:Objective: to compare the contents of total alkaloids and antitussive and anti-inflammatory pharmacological effects between the two new varieties of Fritillaria chinensis, the old varieties of Pinctada chinensis, Pinctada sinensis, and the pharmacological effects of antitussive and anti-inflammatory, and to explore the feasibility of the new source of Fritillaria chinensis.Methods: total alkaloids were extracted by reflux method, and their contents were determined by ultraviolet spectrophotometry, and the pharmacological effects of total alkaloids of Fritillaria japonicus on cough model induced by ammonia stimulation and auricular swelling induced by xylene were compared.The cough inhibition rate and inflammatory inhibition rate of total alkaloids in all varieties were analyzed by cluster analysis.Results: the contents of total alkaloids in the extracts of Fritillaria chinensis were in the order of wild, flat, green, Prunus, Artemisia and Fritillaria taibaiensis (cultivated in order of high to low).The total alkaloids of flat Fritillaria, Pine, Prunus, Pinctada rotundii and Fritillaria tabulaeformis (cultivated) can significantly reduce the cough frequency of mouse cough model induced by ammonia stimulation, among which the old variety is better than the old one, and the new variety is superior in reducing the number of coughs.The total alkaloids of green shellfish can prolong the latent period of cough.The total alkaloids of Fritillaria pinellifolia can obviously reduce the swelling degree of mouse auricle swelling induced by xylene, and the anti-inflammatory effect is better.The results of cluster analysis showed that the total alkaloids and anti-inflammatory effects of total alkaloids in different varieties of Fritillaria paniculata were different from those of Phyllostachys taibaiensis (cultivated and wild), and separated from Ping-Fritillaria chinensis (wild), which indicated that there was a certain difference in the antitussive and anti-inflammatory effects of total alkaloids between different varieties of Fritillaria chinensis.Conclusion: the composition of total alkaloids, anti-inflammatory and antitussive effects of Fritillaria pingbergii are different from those of Fritillaria chinensis, but they can also be used as candidate varieties for further study.
【作者单位】：成都中医药大学;川北医学院;
【基金】：四川省科技支撑项目——中药种质资源库关键技术及示范(2014SZ0156) 中药资源四川省青年科技创新研究团队(2015500028)
【分类号】：O657.3;R284.1;R285.5

【相似文献】