白酒中糖精钠添加剂表面增强拉曼光谱快速检测研究

发布时间：2018-04-04 08:20

本文选题：白酒　切入点：糖精钠　出处：《光谱学与光谱分析》2017年05期

【摘要】：采用表面增强拉曼光谱技术快速分析白酒中非法添加物糖精钠甜味剂。以金溶胶为增强基底,对金溶胶、待测溶液与氯化钠溶液体积比、混合时间和测定体系pH值试验条件进行优化。结果表明,当待测溶液、增强基底和氯化钠溶液体积比为1∶1∶0.5、混合时间为5min、pH值为4时,获得的拉曼信号最好。结合密度泛函理论,对糖精钠分子进行谱峰归属,确定了定性定量分析白酒中糖精钠的3个特征谱峰:1 148,1 164和1 296cm-1。以特征峰1 164cm-1的峰强度与白酒中糖精钠浓度建立线性方程,该方程在1~20mg·L-1浓度范围内线性关系良好,R2决定系数为0.993 3,方法回收率在98.57%~102.5%之间,相对标准偏差(RSD)在2.44%~8.6%之间。此方法分析白酒中糖精钠的最低检出浓度可达到1mg·L-1,单个样本检测时间在10min内完成。研究表明,采用表面增强拉曼光谱方法能快速准确分析白酒中的糖精钠甜味剂,可为白酒中甜味剂实时快速检测装置开发提供方法支持。
[Abstract]:By surface enhanced rapid analysis of illegal additives in liquor sodium saccharin sweeteners Raman spectroscopy. Using gold sol as substrate of colloidal gold test solution with Sodium Chloride Solution volume ratio, mixing time and test conditions for the determination of pH value of the system was optimized. The results show that when the solution to be measured, enhanced basal and Sodium Chloride Solution volume ratio of 1: 1: 0.5, the mixing time is 5min, the pH value is 4, the Raman signal obtained. The best combination of density functional theory, resonance peaks of saccharin molecules, to determine the qualitative and quantitative analysis of 3 characteristics of sodium saccharin in liquor peak: 1 148,1 164 and 1 296cm-1. in linear equations of saccharin sodium the concentration of peak intensity and peak of 1 164cm-1 liquor, the 1~20mg - L-1 equation in the linear range of concentration of R2, the coefficient of determination was 0.9933, the recovery rate is 98.57%~102.5%, the relative standard deviation (RSD) in 2.44%~8.6%. The minimum detectable concentration is 1mg / L-1 sodium saccharin in liquor to the analysis of a single sample, the detection time within 10min. The results show that the surface enhanced Raman spectroscopy method can fast and accurate analysis of sodium saccharin sweeteners in liquor, can provide methodological support for the liquor of sweetener in rapid real-time detection device.

【作者单位】：江西农业大学食品科学与工程学院;江西省出入境检验检疫局技术中心;国家食品安全风险评估中心;江西农业大学生物光电及应用重点实验室;
【基金】：国家“十二五”科技支撑计划项目(2012BAK17B02) 江西省科技支撑计划项目(20151BBG70066)资助
【分类号】：O657.37;TS262.3

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