基于光热-激光背向散射干涉的生物分子定量检测技术

发布时间：2018-04-08 19:10

本文选题：分析化学　切入点：光热效应　出处：《中国科技论文》2017年24期

【摘要】：针对传统的显色法中显色产物的定量检测问题,发展了1种结合光热效应和激光背向散射干涉(back-scattering interferometry,BSI)的生物分子定量检测技术。发生显色反应后的生物分子、离子溶液吸收激光会产生光热效应,使得溶液折射率发生改变,其改变量与待测的分子、离子浓度在一定范围内呈正相关。利用激光背向散射干涉技术可实现折射率变化的灵敏检测,从而实现待测物质浓度的定量检测。文中以亚硝酸盐为例,利用改进的格里斯重氮化反应,使得亚硝酸盐中的NO2-转变为紫红色偶氮化合物。采用波长为532nm的激光,利用显色反应后溶液的光热效应和BSI技术实现了NO2-质量浓度的定量检测,使用3σ方法得到检测限为0.05mg/L。
[Abstract]:In order to solve the problem of quantitative detection of color products in traditional color rendering methods, a biomolecular quantitative detection technique combined with photothermal effect and laser backscattering interferometry (BSI) was developed.After color reaction, laser absorption of ion solution will produce photothermal effect, which changes the refractive index of solution. The change amount is positively correlated with the concentration of ion in a certain range.The laser backscattering interferometry can be used to detect the refractive index change sensitively, thus the quantitative measurement of the concentration of the substance to be measured can be realized.In this paper, nitrite was used as an example to transform no _ 2- in nitrite into a fuchsia azo _ 2 compound by using the improved Gris-diazotization reaction.Using 532nm laser, the photothermal effect of chromogenic solution and BSI technique were used to measure the mass concentration of no _ 2- quantitatively. The detection limit was 0.05 mg / L by using 3 蟽 method.
【作者单位】：太原理工大学新型传感器与智能控制教育部与山西省重点实验室;太原理工大学物理与光电工程学院;
【基金】：国家自然科学基金资助项目(11504259,21505098,21575098) 山西省国际合作资助项目(2015081019) 山西省高等学校科技创新资助项目(2015123) 山西省平台基地和人才专项资助项目(201605D211033)
【分类号】：O657

【相似文献】