吡唑羧酸类铜、锰配合物结构和生物活性研究

发布时间：2018-04-09 06:24

  本文选题：过渡金属配合物　切入点：含氮羧酸配体　出处：《沈阳化工大学》2017年硕士论文

【摘要】：基于晶体工程的金属超分子结构设计和构造目前在超分子化学和材料科学领域中引起了广泛的兴趣。不仅因为他们独特的结构,还有他们的潜在应用,例如发光,催化和生物学应用等,尤其是在现代药物学方面。在合理设计和控制合成这些功能化合物方面,多齿配体如多羧酸类和N-杂环配体得到了广泛的应用。近期,N-杂环羧酸类配体在合成中应用十分广泛,这是由于他们极其丰富的配位模式和功能特性,还有在溶液条件下作为氢键的给予体和受体的特点。过渡金属配合物因其多样的应用,如序列特异性结合,结构探针和治疗药剂,在核酸化学中起到了重要作用。在过去的几十年中,越来越多的含有过渡金属离子如铂和钌的配合物表现出来了有效的抗癌活性。但这些药物具有严重的缺点,对生物靶标的选择性差且具有高毒性。为了找到更好的非铂基金属配合物,我们合成了具有生物抗癌活性的配合物。1,4-双(吡唑-1-基)对苯二甲酸是一种含有多个配位点的新型含氮羧酸配体,因而我们采用它合成了两种新型过渡金属配合物并进行了表征。本文研究的具体内容主要包括以下方面:1、通过水热方法分别制备了铜(Ⅱ)、锰(Ⅱ)配合物的单晶并采用X-射线单晶衍射对配合物进行了结构的测定,并通过红外光谱、元素分析进行了表征。2、采用紫外光谱法和荧光光谱法探究了铜(Ⅱ)、锰(Ⅱ)配合物同DNA的作用方式。通过琼脂糖凝胶电泳测定了配合物对pBR322 DNA具有一定的切割能力。3、采用倒置荧光显微镜观察了铜(Ⅱ)、锰(Ⅱ)配合物对HeLa细胞的作用效果,采用流式细胞仪测定了铜(Ⅱ)、锰(Ⅱ)配合物对KB细胞的杀伤能力。4、采用分子对接软件YASARA进行了铜(Ⅱ)、锰(Ⅱ)配合物与蛋白质的模拟对接,观察了其结合模式。
[Abstract]:The design and construction of metallic supramolecular structures based on crystal engineering have attracted extensive interest in the field of supramolecular chemistry and material science.Not only because of their unique structure, but also because of their potential applications, such as luminescence, catalysis and biological applications, especially in modern pharmacology.Polydentate ligands such as polycarboxylic acids and N-heterocyclic ligands have been widely used in the design and control of these functional compounds.Recently, N-heterocyclic carboxylic acid ligands have been widely used in the synthesis because of their rich coordination patterns and functional characteristics, as well as the characteristics of donors and receptors as hydrogen bonds in solution.Transition metal complexes play an important role in nucleic acid chemistry because of their various applications, such as sequence specific binding, structural probes and therapeutic agents.In the past few decades, more and more complexes containing transition metal ions such as platinum and ruthenium have shown effective anticancer activity.However, these drugs have serious disadvantages, poor selectivity to biological targets and high toxicity.In order to find a better non-platinum metal complex, we synthesized a new nitrocarboxylic acid ligand with biological anticancer activity, I. e., 1,4-bis (pyrazolyl) terephthalic acid.Therefore, two new transition metal complexes were synthesized and characterized.The main contents of this paper are as follows: 1. The single crystals of copper (鈪，

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