基于BODIPY的次氯酸根荧光探针及pH和光双控药物传输纳米硅球的合成与应用研究

发布时间：2018-04-15 18:15

本文选题：荧光探针 + 次氯酸根　；参考：《上海师范大学》2017年硕士论文

【摘要】：本文主要分为两个部分:以氟硼二吡咯(BODIPY)为荧光团、肼基为测试基团,基于自由基反应机理,用于检测次氯酸根离子的高灵敏性、高选择性荧光探针;以无机介孔硅纳米材料(MCM-41)为基底材料,通过表面修饰,实现pH与光照双重控制的药物传输体系。⑴第一部分。由于人体内次氯酸根离子的浓度与人体健康息息相关,故发展检测次氯酸根离子的高选择性、高灵敏性荧光探针引起了人们的广泛关注。本文合成了一种以氟硼二吡咯(BODIPY)为荧光团、以肼基作为次氯酸根检测基团,用于检测次氯酸根离子的新型荧光探针,并对其性质做了探讨。该探针与次氯酸钠的反应在磷酸盐缓冲液中一分钟之内即可完成,反应完成后体系的荧光强度急剧增加。同时,该探针表现出对次氯酸根离子的高选择性和高灵敏度。高效液相色谱、核磁共振氢谱和质谱的数据分析表明,次氯酸根与该探针的反应极有可能是通过自由基氧化机理完成的。⑵第二部分。目前,药物控释体系在医药研发以及实际应用等方面备受人们的青睐。在本文中,我们制备了一种基于介孔纳米硅材料MCM-41,pH和光照双重控制的药物传输体系。该体系分别将氨基硅源与偶氮苯衍生物修饰于MCM-41表面,其中通过对氨基硅源进行修饰并与乙二胺-β-环糊精形成亚胺键来作为pH控制部件;偶氮苯衍生物与β-环糊精形成分子复合体作为光照控制部件。两个控制部件通过一个β-环糊精分子联结为一体。光照控制部件经紫外线照射(365nm)会被打开;pH控制部件在酸性环境中(磷酸盐缓冲液,pH=5.0)会被打开。然而此时材料孔道周围的β-环糊精并未离去,其将继续阻止载体分子释放。只有在酸性环境中(磷酸盐缓冲液,pH=5.0)且有紫外光(365 nm)照射时,pH控制部件和光照控制部件会同时打开,β-环糊精离开材料孔道周围,载体分子大量释放出来。实验结果表明,通过控制纳米门的开/关,能够控制载体部分地释放。如果将材料的每个孔道看做一所房子,β-环糊精看做房子上的一道门,pH控制部件和光照控制部件看做门上的两把锁,那么,只有在同时拥有两把钥匙时才能将这道门打开,将房子里的货物搬出来。如此设计是为了更加精确地控制载体从材料中释放出来,降低药物在到达病变部位之前对正常组织所产生的毒副作用。
[Abstract]:This paper is mainly divided into two parts: fluoroborboron dipyrrolidine (BODIPY) as fluorescence group, hydrazine group as test group, based on the mechanism of free radical reaction, used for the detection of hypochlorite ion high sensitivity, high selectivity fluorescence probe;Using inorganic mesoporous silicon nanomaterials (MCM-41) as substrate, the drug transport system with double pH and illumination was realized by surface modification.Because the concentration of hypochlorite ion in human body is closely related to human health, the development of the detection of hypochlorite ion high selectivity, high sensitivity fluorescent probe has attracted people's attention.In this paper, a new fluorescent probe for the detection of hypochlorite ions using fluoroborate dipyrrolidine (BODIPY) as fluorescence group and hydrazine as detection group was synthesized, and its properties were discussed.The reaction between the probe and sodium hypochlorite was completed within one minute in the phosphate buffer, and the fluorescence intensity of the system increased sharply after the reaction was completed.At the same time, the probe showed high selectivity and high sensitivity to hypochlorite ions.High performance liquid chromatography (HPLC), nuclear magnetic resonance spectroscopy (NMR) and mass spectrometry (MS) showed that the reaction of hypochlorite with the probe was most likely due to the second part of the radical oxidation mechanism.At present, drug-controlled release system is very popular in pharmaceutical research and development as well as practical application.In this paper, we have prepared a drug transport system based on the double control of pH and illumination of mesoporous nanocrystalline silicon (MCM-41).In this system, the amino silicon source and azobenzene derivatives were modified on the surface of MCM-41, in which the amino silicon source was modified and the imine bond was formed with ethylenediamine- 尾 -cyclodextrin as the pH control component.Azobenzene derivatives and 尾-cyclodextrin form molecular complexes as light control parts.Two control components are bound together by a 尾-cyclodextrin molecule.The light control unit was exposed to ultraviolet light (365 nm) and the pH control unit was opened in acidic environment (phosphate buffer pH 5.0).At this time, however, the 尾-cyclodextrin around the pore does not leave, and it will continue to block the release of the carrier molecule.Only in acidic environment (phosphate buffer pH 5.0 and UV irradiation 365 nm) will the pH control component and the light control component open simultaneously, and 尾 -cyclodextrin leave the pore of the material and the carrier molecules will be released in large quantities.The experimental results show that the partial release of the support can be controlled by controlling the opening / closing of the nanogates.If you look at each hole in the material as a house, 尾 -cyclodextrin as a door pH control component and a light control component as two locks on the door, then you can only open the door if you have two keys at the same time.Move the goods out of the house.This design is designed to more precisely control the release of the carrier from the material to reduce the toxic side effects of the drug on normal tissue before reaching the diseased site.
【学位授予单位】：上海师范大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：O657.3;TQ460.1

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