高效液相色谱与纳流电喷雾阵列Chirp Z变换离子迁移谱联用方法研究

发布时间：2018-04-15 17:33

本文选题：Chirp + Z变换　；参考：《分析化学》2017年06期

【摘要】：利用柱后分流频率调制Chirp Z变换离子迁移谱(IMS),建立了高效液相色谱与纳流电喷雾阵列Chirp Z变换离子迁移谱(HPLC-Nano ESI-Chirp Z IMS)联用分析方法,考察了喷雾电压、溶剂组成、溶液流速等参数对Nano ESI-Chirp Z IMS信噪比的影响。在此基础上,利用建立的方法对一系列四烷基溴化铵类化合物进行测定,并比较了Chirp Z变换法和FT变换法两种方法的信噪比和分辨率。实验结果表明,最佳实验条件为:喷雾电压4.5 k V;溶剂组成90%甲醇;ESI溶液流速为8μL/min。利用HPLC-Nano ESI-Chirp Z IMS联用方法成功测定了四丁基溴化铵、四戊基溴化铵、四己基溴化铵、四庚基溴化铵、四辛基溴化铵烷、四癸基溴化铵组成的混合样品。与传统信号平均离子迁移谱相比,Chirp Z变换离子迁移谱的信噪比更高,其迁移时间的测定精度优于傅里叶变换离子迁移谱。
[Abstract]:A method for the analysis of HPLC-Nano ESI-Chirp Z IMSs by Chirp Z shift ion transfer spectrum (HPLC-Nano ESI-Chirp Z IMSs) was established. The spray voltage and solvent composition were investigated. The method was used to analyze the HPLC-Nano ESI-Chirp Z IMSs by using high performance liquid chromatography (HPLC) and nanoscale electrospray array (Chirp Z transform ion transfer spectrum).Effect of flow rate of solution on SNR of Nano ESI-Chirp Z IMS.On this basis, a series of tetraalkylammonium bromide compounds were determined by the established method, and the signal-to-noise ratio (SNR) and resolution of the two methods were compared between the Chirp Z transform method and the FT transform method.The experimental results show that the optimum experimental conditions are as follows: spray voltage 4.5 kV, solvent composition 90% methanol ESI solution flow rate 8 渭 L / min.The mixed samples of tetra, tetraamyl ammonium bromide, tetrahexyl ammonium bromide, tetraheptyl ammonium bromide, tetraoctyl ammonium bromide and tetradecyl ammonium bromide were determined successfully by HPLC-Nano ESI-Chirp Z IMS.The SNR of Chirp Z transform ion transport spectrum is higher than that of conventional average ion transport spectrum, and the measurement accuracy of the migration time is better than that of Fourier transform ion transport spectrum.
【作者单位】：塔里木大学生命科学学院;新疆生产建设兵团塔里木盆地生物资源保护利用重点实验室;
【基金】：兵团国际合作项目(No.2015AH005) 研究生创生项目(No.TDGRI 201523) 国家自然科学基金项目(No.21365018)资助~~
【分类号】：O657

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