气血双补酊剂HPLC指纹图谱及含量测定（英文）

发布时间：2018-04-16 08:03

本文选题：指纹图谱分析 + 高效液相-二极管阵列检测　；参考：《中国实验方剂学杂志》2017年01期

【摘要】：目的:采用HPLC-DAD方法,对气血双补酊剂进行指纹图谱分析和10种活性成分同时测定来进行质量控制。方法:通过优化提取、分离和分析条件,采用Merck C18色谱柱,流动相乙腈-0.1%磷酸水溶液,波长203 nm。结果:通过分析10批样品指纹图谱,确立了32个共有峰作为特征峰来进行评价,10批样品相似度高,10种活性成分含量同时测定的结果稳定可靠。结论:该多成分含量测定的方法,结合色谱指纹图谱分析,具有实际应用,可以全面对气血双补酊剂进行质量进行控制。
[Abstract]:Objective: to analyze the fingerprint of Qi-Xue Shuangbu tincture by HPLC-DAD and determine 10 active components simultaneously to control the quality of tincture.Methods: by optimizing extraction, separation and analysis conditions, Merck C18 column, mobile phase acetonitrile-0.1% phosphoric acid aqueous solution, wavelength 203nm.Results: by analyzing the fingerprint of 10 batches of samples, 32 common peaks were established as characteristic peaks to evaluate 10 batches of samples with high similarity and 10 kinds of active components. The results of simultaneous determination of 10 kinds of active components in 10 batches of samples were stable and reliable.Conclusion: the method of multicomponent determination combined with chromatographic fingerprint analysis has practical application and can be used to control the quality of Qi-Xue Shuangbu tincture.
【作者单位】：河北医科大学第三医院;河北医科大学;
【基金】：河北省自然科学基金项目(H2013206010)
【分类号】：R286.0;O657.72

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