临床血脂干化学检测方法的研究与应用

发布时间：2018-04-19 12:43

本文选题：干片 + 临床检验　；参考：《长春理工大学》2017年硕士论文

【摘要】：随着临床检验技术的快速发展,在要求结果准确稳定的同时,又要加快检验结果的速度,为临床科室提供快速准确的数据。在临床检验科室中,干化学检验技术在生化分析领域与传统湿生化分析领域相比,干化学检验技术具有快速、准确,干片易保存,便于携带,操作检测简单方便等优点。但干化学检验技术在国外应用比较成熟,国内起步较晚,现阶段主要依赖国外成熟干片。因此研发出具有自主知识产权的干式生化检验技术的干片,对推动干式生化分析技术的国产化具有重要意义。本文研究建立三种用于快速检测血液中胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白(HDL-C)含量的干式生化分析方法。按照测试项目筛选出不同干片载体膜材料,制备成干片,用干式生化分析仪检测干片,并建立了测定血液中TC、TG、HDL-C含量的标准曲线,对建立三种干片的方法学进行了线性实验、批间与批内重复性实验。运用此方法制备的干片颜色梯度明显、显色均匀,重复性和稳定性较好,TC、TG、HDL-C的线性范围分别为2.59-9.04 mmol/L、0.565-3.39 mmol/L、0.48-2.88 mmol/L。将制备好的干片置于冰箱4℃条件下,在30天内重复性、稳定性良好;批内与批间重复性变异系数都在5%以下,符合临床检验的要求,与临床湿生化结果对比,结果重复性变异系数小于5%,可应用于临床检测。
[Abstract]:With the rapid development of clinical test technology, the rapid and accurate data are provided for the clinical department, while the results are faster and more accurate. In the clinical laboratory, dry chemical inspection technology is fast and accurate in the field of biochemical analysis compared with the traditional wet biochemical analysis field. Dry film is easy to be preserved, easy to carry and simple and convenient to operate. However, the dry chemical inspection technology is more mature in foreign countries and is late at home. At present, it mainly depends on foreign mature dry tablets. Therefore, dry chemical test technology with independent intellectual property right is developed. It is of great significance. In this paper, three dry biochemical analysis methods used to quickly detect the content of cholesterol (TC), triglyceride (TG) and high density lipoprotein (HDL-C) in blood were developed. In accordance with the test items, the membrane materials of different carrier films were screened, dried slices were prepared, dry chemical analyzer was used to detect dry slices, and the determination of TC, TG, H in blood was established. The standard curve of DL-C content has been carried out in linear experiments and in batch and batch repeatability experiments for the establishment of three kinds of dry slices. The color gradient of the dry slices made by this method is obvious, the color rendering is uniform, and the repeatability and stability are good. The linear range of TC, TG and HDL-C is 2.59-9.04 mmol/L, 0.565-3.39 mmol/L, 0.48-2.88 mmol/L. will be prepared, respectively. Under the condition of 4 degrees fridge, the good dry pieces were repeatable and stable in 30 days. The repeatability coefficient of variation within and between batch and batch was below 5%, which accorded with the requirements of clinical test, compared with the clinical wet biochemical results, the result of repeatability variation coefficient was less than 5%, which could be applied to clinical examination.

【学位授予单位】：长春理工大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R446.1;O652

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