银纳米粒子的可控制备及其表面等离子体共振增强荧光的研究

发布时间：2018-04-21 07:32

本文选题：银纳米粒子 + 动力学　；参考：《青岛科技大学》2017年硕士论文

【摘要】：银纳米材料因其自身具有独特的物理与化学性能,在催化、抑菌、电学、光学等领域有着广泛的应用。银纳米粒子的性质主要取决于它的形貌和尺寸,因此研究银纳米颗粒的可控制备具有重要的意义。在合适的入射光照射下,银纳米粒子表现出优异的表面等离子体共振效应,这种共振效应在荧光、拉曼信号增强方面有着广泛的应用。本文在传统制备银纳米粒子方法的基础上,通过优化实验条件制备了球形、粒径相对均一的银纳米粒子。在此基础上利用银纳米粒子优异的表面等离子共振增强荧光效应,实现对Cu~(2+)、焦磷酸根、凝血酶的灵敏检测。本文主要进行了以下三个方面的研究。1.在传统柠檬酸钠还原硝酸银制备银纳米粒子的基础上,研究了动力学控制银纳米粒子生长的影响因素。通过控制种子量、pH值以及温度,可控得到30-157 nm的类球形银纳米粒子。2.利用银纳米粒子表面等离子体共振增强荧光的特性,通过调控SiO_2壳层的厚度,实现Ag@SiO_2对CdS量子点荧光强度的最大增强,实验表明SiO_2的厚度为14 nm时,荧光强度达到2.3倍最大增强。利用CdS量子点对Cu~(2+)特异性选择及PPi与Cu~(2+)之间具有的较强的结合能力,实现了对Cu~(2+)、焦磷酸根的灵敏检测。3.利用银纳米粒子表面等离子体共振增强荧光的特性,通过DNA碱基互补配对的原则,将有机荧光染料Cy5带到Ag@SiO_2表面,并探究了不同壳层厚度的Ag@SiO_2对荧光材料Cy5荧光强度的增强作用,最终实现了对Cy5荧光强度6.3倍的增强。利用凝血酶与适配体之间特异性结合以及氧化石墨烯与荧光材料Cy5之间的荧光共振能量转移效应,实现了对凝血酶的灵敏检测。
[Abstract]:Silver nanomaterials have been widely used in catalysis, bacteriostatic, electrical and optical fields because of their unique physical and chemical properties. The properties of silver nanoparticles mainly depend on their morphology and size, so it is of great significance to study the controllable preparation of silver nanoparticles. Silver nanoparticles exhibit excellent surface plasmon resonance (SPR) effect under appropriate incident light, which is widely used in fluorescence and Raman signal enhancement. Based on the traditional method of preparing silver nanoparticles, spherical and relatively homogeneous silver nanoparticles were prepared by optimizing the experimental conditions. On this basis, the excellent surface plasmon resonance enhanced fluorescence effect of silver nanoparticles was used to realize the sensitive detection of Cu~(2, pyrophosphate and thrombin. This paper mainly carries on the following three aspects of research. 1. Based on the reduction of silver nitrate by sodium citrate to silver nanoparticles, the factors influencing the growth of silver nanoparticles were studied. By controlling the amount of seed pH and temperature, the spherical silver nanoparticles of 30-157 nm were obtained. Using the characteristics of silver nanoparticles surface plasmon resonance enhanced fluorescence, the maximum fluorescence intensity of Ag@SiO_2 on CdS quantum dots is realized by adjusting the thickness of SiO_2 shell. The experimental results show that the thickness of SiO_2 is 14 nm. Fluorescence intensity reached 2.3 times maximum enhancement. The specific selection of Cu~(2 by CdS quantum dots and the strong binding ability between PPi and Cu~(2) were used to realize the sensitive detection of Cu~(2 and pyrophosphate. Based on the surface plasmon resonance enhanced fluorescence of silver nanoparticles and the principle of complementary matching of DNA bases, the organic fluorescent dye Cy5 was brought to the surface of Ag@SiO_2. The enhancement effect of Ag@SiO_2 with different shell thickness on the fluorescence intensity of Cy5 was investigated. Finally, the fluorescence intensity of Cy5 was enhanced by 6.3 times. The specific binding between thrombin and aptamer and the fluorescence resonance energy transfer effect between graphene oxide and fluorescent material Cy5 were used to detect thrombin sensitively.
【学位授予单位】：青岛科技大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：TB383.1;O657.3

【参考文献】