超高效液相色谱法双波长法同时测定二妙丸中5种成分的含量

发布时间：2018-04-22 09:04

  本文选题：超高效液相色谱法双波长法 + 含量测定　； 参考：《中国药学杂志》2017年23期

【摘要】：目的建立二妙丸中盐酸小檗碱、盐酸黄柏碱、盐酸巴马汀、木兰花碱及苍术素等5种成分的含量测定方法。方法采用超高效液相色谱法,色谱柱:Waters BEH(2.1 mm×100 mm,1.7μm),乙腈-0.1%磷酸(每100 m L加十二烷基硫酸钠0.1 g)为流动相,梯度洗脱,流速为0.3 m L·min~(-1),盐酸黄柏碱、木兰花碱、盐酸小檗碱、巴马汀检测波长为280 nm;苍术素检测波长为340 nm;柱温:30℃。结果盐酸小檗碱、黄柏碱、巴马汀、木兰花碱及苍术素5种成分在一定范围内浓度与峰面积呈良好的线性关系;精密度,稳定性,重现性等均符合方法学要求;平均回收率在96.8%~99.3%之间,RSD均小于2.8%。结论建立的方法简便快捷,准确度高,重现性好,可为二妙丸的质量控制提供依据。
[Abstract]:Objective to establish a method for the determination of berberine hydrochloride, berberine hydrochloride, palmatine hydrochloride, Magnoline and Atractylosin in Ermiao pills. Methods Ultra-high performance liquid chromatography was used. The flow rate was 0.3 mL 路min ~ (-1), the flow rate was 0.3 mL 路min ~ (-1), the flow rate was 0.3 mL 路min ~ (-1) ~ (-1), the flow rate was 0.3 mL 路min ~ (-1), the flow rate was 0.3 mL 路min ~ (-1), the flow rate was 0.3 mL 路min ~ (-1) ~ (-1), the flow rate was 0.3 mL 路min ~ (-1) ~ (-1). The detection wavelength of Bamatin was 280 nm, the detection wavelength of Atractylodes macrocephala was 340 nm, the column temperature was 30 鈩，

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