谷胱甘肽比率荧光探针分子的设计合成及其光谱性能

发布时间：2018-04-27 03:06

本文选题：谷胱甘肽 + 比率荧光探针　；参考：《发光学报》2017年12期

【摘要】：将喹啉和丹磺酰胺两种荧光基团同时引入配体L1,利用L1与锌离子自组装构筑三核锌有机-金属大环化合物H-1(比率荧光探针),实现了对生物分子谷胱甘肽(GSH)的有效识别。利用紫外光谱、荧光光谱、~1H NMR、ESI-MS等表征方法研究了H-1对生物分子谷胱甘肽(GSH)的光谱识别作用。紫外滴定光谱表明,当向H-1中加入谷胱甘肽分子后,425 nm处的吸收峰强度降低,320 nm处的吸收峰强度增大,等吸收点为355 nm。利用320 nm处的吸光度值模拟计算平衡常数,lg K为4.03±0.11,说明H-1与GSH形成了1∶1的包合物。荧光光谱分析表明,当向H-1中加入GSH后,以340 nm光激发,波长为513 nm处丹磺酰胺的荧光强度下降,并且发生红移,而396 nm处喹啉基团的荧光强度增大。利用喹啉基团与丹磺酰胺基团荧光发射峰强度变化的比值可以精准检测谷胱甘肽分子,检测限可达到2.5×10~(-6)mol·L~(-1)。
[Abstract]:Two fluorescent groups, quinoline and dansulfonamide, were introduced into ligand L1 at the same time, and trinuclear organo-metal macrocyclic compound H-1 (ratio fluorescence probe) was constructed by self-assembly of L1 and zinc ions. The effective recognition of glutathione (GSH) was achieved. The spectral recognition of glutathione glutathione (GSH) by H-1 was studied by UV and fluorescence spectra. The UV titration spectra show that the absorption peak intensity at 425 nm decreases and increases at 320 nm when glutathione molecule is added to H-1, and the isoabsorption point is 355 nm. The equilibrium constant K is 4.03 卤0.11 by using the absorbance at 320 nm, which indicates that the inclusion compound between H-1 and GSH is 1:1. Fluorescence spectrum analysis showed that when GSH was added to H-1, the fluorescence intensity of dansulfonamide decreased at 513 nm and red shifted, but the fluorescence intensity of quinoline group increased at 396nm. Using the ratio of quinoline group to Dansulfonamide group fluorescence emission peak intensity, glutathione molecule can be detected accurately, and the detection limit can reach 2.5 脳 10~(-6)mol / L ~ (-1) ~ (-1).
【作者单位】：辽宁工业大学化学与环境工程学院;
【基金】：国家自然科学基金(21601075) 辽宁省自然科学基金(2015020249)资助项目~~
【分类号】：O657.3

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