杂色曲霉毒素印迹聚离子液体材料制备及分离性能研究

发布时间：2018-04-27 03:24

本文选题：杂色曲霉毒素 + 离子液体　；参考：《天津科技大学》2017年硕士论文

【摘要】：杂色曲霉毒素(ST)是一种霉菌毒素,主要是由杂色曲霉、构巢曲霉和离孺孢霉等真菌产生的有毒次级代谢产物,广泛存在于自然界中,主要对小麦、玉米、花生、大豆等粮食作物及动物饲料造成污染,是最常见的霉菌毒素污染物之一,人和畜禽摄入含有杂色曲霉毒素的食物会引起广泛的毒性效应。所以建立杂色曲霉毒素的检测方法具有重要意义。本论文合成了离子液体1-乙烯基-3-丙胺咪唑氢溴酸盐和1-乙烯基-3-丁烯咪唑溴盐,分别作为印迹过程中的功能单体和交联剂,制备了两种对ST具有选择性的分子印迹聚合物。实验以目标物结构类似物1,8-二羟基蒽醌作为ST替代模板,1-乙烯基-3-丙胺咪唑氢溴酸盐作为功能单体,1-乙烯基-3-丁烯咪唑溴盐作为交联剂,偶氮二异丁腈为引发剂,采用原位聚合法成功制备了对ST具有特异性吸附的印迹聚离子液体毛细管整体柱。以1,8-二羟基蒽醌作为ST的替代模板,1-乙烯基-3-丙胺咪唑氢溴酸盐作为功能单体,1-乙烯基-3-丁烯咪唑溴盐作为交联剂,偶氮二异丁腈为引发剂,采用本体聚合法制备了对ST具有特异性吸附性能的印迹聚离子液体聚合物,并将该分子印迹聚合物作为固相萃取的填充材料。采用原位聚合法直接在毛细管柱中合成出具有空穴结构的毛细管分子印迹整体柱。通过扫描电镜表征显示,所制备的毛细管整体柱内部结构均匀,孔隙度适中。采用电色谱模式对杂色曲霉毒素和其结构类似物黄曲霉毒素B1进行了分离。通过对分离条件的优化实现了杂色曲霉毒素和黄曲霉毒素B1的快速分离。通过本体聚合法制备的印迹聚离子液体聚合物,利用扫描电镜对制备的印迹聚离子液体聚合物进行了结构形态表征,通过吸附实验进行了吸附性能表征。实验结果表明,制备的聚离子液体印迹聚合物对杂色曲霉毒素具有较好的吸附效果。将聚离子液体印迹聚合物作为固相萃取材料,建立了杂色曲霉毒素分子印迹固相萃取与高效液相色谱联用的方法,并应用于对实际样品的检测。
[Abstract]:Aspergillus chromotoxin (STT) is a mycotoxin, mainly a toxic secondary metabolite produced by Aspergillus heterochromoides, Aspergillus monocystis and Aspergillus sp., and other fungi. It is widely found in nature, mainly in wheat, corn, peanut, etc. Soybean and other food crops and animal feed pollution is one of the most common mycotoxin pollutants human and poultry intake of food containing aspergillus mutans can cause a wide range of toxic effects. Therefore, it is of great significance to establish a method for the detection of aspergillus mutans. In this paper, ionic liquids 1-vinyl-3-propylaminoimidazole hydrobromide and 1-vinyl-3-butene imidazolium bromide were synthesized. As functional monomers and crosslinkers in the imprinting process, two kinds of molecularly imprinted polymers with selectivity to St were prepared. In this experiment, the target structure analogue, 1o 8-dihydroxyanthraquinone, was used as a substitute for St template, 1-vinyl-3-propylaminoimidazole hydrobromate as the functional monomer 1-vinyl-3-butylene imidazolium bromide as crosslinking agent, and azodiisobutyronitrile as initiator, and azodiisobutyronitrile as initiator. Imprinted polyionic liquid capillary monolithic column with specific adsorption of St was successfully prepared by in situ polymerization. 1-vinyl-3-propylaminoimidazolium hydrobromide was used as a functional monomer, 1-vinyl-3-butenyl imidazolium bromide as crosslinking agent and azodiisobutyronitrile as initiator. The imprinted polyionic liquid polymer with specific adsorption property for St was prepared by bulk polymerization, and the molecularly imprinted polymer was used as the filling material for solid phase extraction (SPE). A capillary molecularly imprinted monolithic column with hole structure was synthesized by in-situ polymerization. Scanning electron microscopy (SEM) showed that the capillary column had a uniform internal structure and a moderate porosity. The hybrid aspergillus toxin and its structural analogue aflatoxin B1 were separated by electrochromatography. The rapid separation of aflatoxin and aflatoxin B1 was achieved by optimizing the separation conditions. The imprinted polyionic liquid polymer was prepared by bulk polymerization. The structure and morphology of the imprinted polyionic liquid polymer were characterized by scanning electron microscope. The adsorption properties of the imprinted polyionic liquid polymer were characterized by adsorption experiments. The experimental results show that the polyionic liquid imprinted polymer has a good adsorption effect on the heterochromatic aspergillus toxin. The polyionic liquid imprinted polymer was used as solid phase extraction material to establish the method of molecular imprinted solid phase extraction of aspergillus chromotoxin and high performance liquid chromatography (HPLC), and the method was applied to the detection of real samples.
【学位授予单位】：天津科技大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：TS207.5;O658.2

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