居间驹形氏杆菌发酵大豆糖蜜生产细菌纤维素条件的优化

发布时间：2018-04-27 10:33

  本文选题：大豆糖蜜 + 细菌纤维素　； 参考：《食品科学》2017年18期

【摘要】：通过居间驹形氏杆菌(Komagataeibacter intermedius CGMCC12562)发酵生产细菌纤维素,以大豆糖蜜作主要发酵原料,优化发酵工艺参数,提高细菌纤维素产量。在单因素试验的基础上,筛选出对细菌纤维素产量影响较大的4个因素,即大豆糖蜜可溶性固形物含量、玉米蛋白粉、ZnSO_4及苹果酸添加量,并通过Design-Expert响应面分析对这4个因素进行优化,得到最优的培养基及发酵工艺为:大豆糖蜜可溶性固形物含量14.13°Brix、玉米蛋白粉添加量1.6%、ZnSO_4添加量0.11%、苹果酸添加量0.41%;在30℃、接种量10%、装料量16%、初始pH 6.0的条件下静置培养7d,细菌纤维素产量高达(15.68±0.82)g/L。
[Abstract]:Bacterial cellulose was produced by the fermentation of Komagataeibacter intermedius CGMCC12562. Soybean molasses was used as the main fermentation material to optimize the fermentation parameters and increase the production of bacterial cellulose. On the basis of single factor experiment, four factors were selected to influence the yield of bacterial cellulose, namely, the content of soluble solids in soybean molasses, the content of ZnSO4 in corn protein powder and the amount of malic acid added. The four factors were optimized by Design-Expert response surface analysis. The optimum medium and fermentation technology were obtained as follows: the content of soluble solids in molasses of soybean was 14.13 掳Brix, the amount of corn protein powder was 1.6 and the amount of ZnSO4 was 0.11 and the amount of malic acid was 0.41 at 30 鈩，

本文编号：1810376