PLGA-磷酸钙复合载药纳米粒的制备

发布时间：2018-04-27 13:36

本文选题：可降解高分子 + 磷酸钙　；参考：《高分子学报》2016年10期

【摘要】：利用聚氧乙烯硬脂酸酯(Brij78)和帕米膦酸二钠制备新型表面活性剂Pa-Brij78,以此为表面活性剂,聚乳酸-羟基乙酸共聚物(poly(lactic-co-glycolic acid),PLGA)为油相,采用水包油包水的微乳液法制备载卵清蛋白(ovalbumin,OVA)的表面带有磷酸根的PLGA纳米粒,再用共沉淀法在其表面修饰一层磷酸钙,并装载寡核苷酸Cp G,形成一种核-壳结构的复合载药纳米粒Cp G/Ca P/PLGA(OVA).通过动态光散射粒度仪、扫描电子显微镜(SEM)、透射电子显微镜(TEM)、X射线衍射(XRD)对纳米粒进行表征,并测定OVA、Cp G的载药量、包封率.结果表明以Pa-Brij78为表面活性剂制备的PLGA(OVA)纳米粒确实能被磷酸钙修饰,粒径增大40~60 nm,表面变粗糙,XRD测得该磷酸钙层的主要存在形式为Ca3(PO4)2.OVA平均载药量为5%,包封率大于80%;Cp G平均载药量为0.47%,平均包封率为89.9%.
[Abstract]:A new surfactant Pa-Brij78 was prepared from polyoxyethylene stearate (Brij78) and pamiphosphonate disodium. The new surfactant Pa-Brij78 was used as surfactant, and polylactic acid-co-glycolic acid (PLGA) was used as oil phase. PLGA nanoparticles with phosphate on the surface of ovalbumin ovalbumin ovalbumin OVA were prepared by water in water microemulsion method. The surface of the nanoparticles was modified with calcium phosphate by co-precipitation method. And the oligonucleotide CP G was loaded to form a core-shell composite drug loaded nanoparticles CP G/Ca P / PLGA OVA. The nanoparticles were characterized by dynamic light scattering particle size analyzer, scanning electron microscope (SEM) and transmission electron microscope (TEM). The results showed that PLGA OVA nanoparticles prepared with Pa-Brij78 as surfactant could be modified by calcium phosphate. The main forms of the calcium phosphate layer are: the average drug loading of Ca3(PO4)2.OVA is 5, the entrapment efficiency is greater than 80% CP G is 0.47 and the average entrapment efficiency is 89. 9%.
【作者单位】：西南交通大学生命科学与工程学院;西南交通大学材料科学与工程学院;
【基金】：四川省科技厅项目(项目号2015JY0202) 国家自然科学基金(基金号21574105) 四川省高校创新研究团队建设项目(项目号14TD0050) 成都市科技惠民项目(项目号2015-HM01-00047-SF)资助
【分类号】：O631;TQ460.1

【相似文献】