HPLC法测定仿野生栽培灵芝中多糖肽和灵芝酸含量

发布时间：2018-05-01 03:31

本文选题：仿野生栽培灵芝 + 灵芝多糖肽　；参考：《基因组学与应用生物学》2017年11期

【摘要】：本研究目的是探讨仿野生栽培灵芝的多糖肽和灵芝酸含量变化,为规范灵芝栽培及产品质量提供依据。本研究采用RP-HPLC测定灵芝酸含量和HPLC-ELSD法测定灵芝多糖肽含量。结果显示:(1)低分子物质(以灵芝酸A+B+C2计):菌蕾0.52%,菌盖0.25%,菌柄0.13%,菌盖比菌柄高48%;(2)多糖肽提取得率比较:菌蕾提取得率为2.12%,菌柄0.70%,菌盖1.23%;(3)含量(以GLPPSQ计)比较:菌蕾1.30%,菌盖0.3%,菌柄0.2%;(4)以上醇溶性灵芝酸和水溶性多肽结果:菌蕾菌盖菌柄。上述结果说明仿野生栽培灵芝不同发育期的有效成分提取率和含量都存在明显差异,这对灵芝的合理应用提供一定的科学依据。
[Abstract]:The purpose of this study was to investigate the changes of polyglycopeptide and ganodermic acid content of Ganoderma lucidum in wild cultivation, and to provide the basis for regulating the cultivation and product quality of Ganoderma lucidum. In this study, the content of Ganoderma lucidum polysaccharide peptide and the content of Ganoderma lucidum polysaccharide peptide were determined by RP-HPLC and HPLC-ELSD. The results showed that the extraction rate of polyglycopeptide was 2.12%, 0.70%, 0.70% and 1.23% (GLPPSQ) of low molecular weight (A / B C2 Ganoderic acid: 0.52%, 0.25%, 0.13%, 48% and 2% respectively) (in terms of GLPPSQ). The results showed that the content of polyglycopeptide was 2.12%, 0.70% and 1.23% respectively (in GLPPSQ). The results showed that the extraction rate of polyglycopeptide was 2.12%, 0.70% and 1.23% (in GLPPSQ). The results of alcohol soluble ganodermic acid and water-soluble polypeptide above 1.30%, 0.3% and 0.2% (P < 0.05): the stem of Ganoderma lucidum and water soluble polypeptide. The results showed that the extraction rate and content of effective components of Ganoderma lucidum in different developmental stages were obviously different, which provided a scientific basis for the rational application of Ganoderma lucidum.
【作者单位】：福建农林大学食品科学学院;国家菌草工程技术研究中心;儋州牧春绿色生态农业开发有限公司;福建松溪县仙草坊专业合作社;福建农林大学动物科学学院;
【基金】：菌草生态产业协同创新中心(K80ND8002) 海南省应用技术研发与示范推广专项(ZDXM2015132)共同资助
【分类号】：O657.72;R284.1

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