三种多环芳烃混合溶液二维荧光相关谱解析

发布时间：2018-05-01 05:05

本文选题：多环芳烃 + 二维荧光相关　；参考：《光谱学与光谱分析》2016年02期

【摘要】：多环芳烃为优先控制污染物,但是由于其含量很低,多组分多环芳烃荧光峰相互重叠,所以常规荧光光谱法无法对其荧光峰进行有效解析。采用二维荧光相关分析方法对三种多环芳烃,蒽、菲和芘的混合溶液进行荧光峰解析。根据研究目标,按照三种多环芳烃浓度比的不同配制了三种混合物体系,共27个样本,每种体系的三种溶液浓度彼此间按规律递增和递减。在此基础上,以浓度为外扰,构建了各体系的同步和异步二维荧光相关谱。同步谱中,在425,402,381,373,365,393及347nm处产生自相关峰。以未被覆盖的菲在347nm处荧光峰为线索,通过其与各波长处荧光交叉峰的正负,判断出了402,381,425和452nm处荧光峰源于混合溶液中的蒽;373与393nm处荧光峰源于混合溶液中的芘;365,356及347nm处荧光峰源于混合溶液中的菲。通过异步谱解析出菲的385nm处荧光峰,证明了异步谱比同步谱具有更好的光谱分辨率。研究结果表明,采用二维荧光相关方法对光谱严重重叠的多组分多环芳烃的解析是可行的,并具有一定的优势,可推广到对环境中其他污染物质的检测。
[Abstract]:Polycyclic aromatic hydrocarbons (PAHs) are preferred to control pollutants, but due to their low content, the fluorescence peaks of polycyclic aromatic hydrocarbons overlap with each other, so the fluorescence peaks of polycyclic aromatic hydrocarbons can not be effectively resolved by conventional fluorescence spectrometry. Fluorescence peaks of three polycyclic aromatic hydrocarbons (PAHs) anthracene phenanthrene and pyrene were analyzed by two-dimensional fluorescence correlation analysis. According to the research objective, three mixtures were prepared according to the different concentration ratios of three polycyclic aromatic hydrocarbons (PAHs). There were 27 samples. The concentrations of the three solutions in each system increased and decreased according to the law. On this basis, the synchronous and asynchronous two dimensional fluorescence correlation spectra were constructed with the concentration as the external disturbance. In the synchronous spectrum, autocorrelation peaks occur at 425402381373365393 and 347nm. Taking the uncovered phenanthrene fluorescence peak at 347nm as the clue, the fluorescence cross peak at each wavelength is positive or negative. It was found that the fluorescence peaks at 402381425 and 452nm originated from pyrene 365356 in mixed solution and at 393nm from phenanthrene in mixed solution, and the fluorescence peak at 347nm came from phenanthrene in mixed solution. The fluorescence peak at 385nm of phenanthrene is analyzed by asynchronous spectrum, which proves that asynchronous spectrum has better spectral resolution than synchronous spectrum. The results show that the two-dimension fluorescence correlation method is feasible for the analysis of polycyclic aromatic hydrocarbons (PAHs) with serious overlapping spectra, and has some advantages, which can be extended to the detection of other pollutants in the environment.
【作者单位】：天津大学精密测试技术及仪器国家重点实验室;天津农学院工程技术学院;天津农学院农业分析测试中心;
【基金】：天津市自然科学基金项目(14JCYBJC30400) 天津市教委科技发展基金项目(20140621) 国家自然科学基金项目(31201359)资助
【分类号】：O657.3;O625.1

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