免疫分离结合表面增强拉曼光谱检测环境及谷物中的农药残留

发布时间：2018-05-01 16:15

本文选题：阿特拉津 + 莎稗磷　；参考：《天津科技大学》2017年硕士论文

【摘要】：阿特拉津是一种三嗪类农药,莎稗磷是一种有机磷农药,两者均用于抑制农田中杂草的生长,然而农药的不恰当使用产生了农药残留问题,进而对环境及农作物安全甚至人类的健康产生了危害。本实验建立了一种免疫分离结合表面增强拉曼光谱检测环境及谷物中的农药残留的新方法。实验以树枝状纳米银作为拉曼增强基底,以三聚氰胺作为拉曼信号物,标记半抗原。建立了竞争性免疫反应结合表面增强拉曼光谱检测环境及谷物中的农药残留。通过多次实验得到拉曼光谱的最佳扫描参数:扫描时间为10s,扫描次数为3次,激光功率为0.5%-5%。抗体的稀释倍数为10倍稀释,标记半抗原的稀释倍数为100倍稀释,孵育时间为25分钟,PBST缓冲液的洗脱次数为2次。实验选取阿特拉津和莎稗磷两种目标物,以0.01ng/mL-100μg/mL浓度梯度为横坐标,以三聚氰胺信号峰强度为纵坐标,建立标准曲线。得到阿特拉津的标准曲线方程为 y=-4871.6x+17999,R2=0.9705,莎稗磷的标准曲线方程为 y=-7079.3x+26571,R2=0.9688。两种目标物的方法检出限均为0.01ng/mL。通过本实验建立的免疫分离结合表面增强拉曼光谱检测环境及谷物中的两种农残所需检测时间短,且灵敏度高,特异性好,对于实际样品的加标回收率较高,适合多种农药残留的定量检测。
[Abstract]:Atrazine is a triazine pesticide, and paspalum is an organophosphorus pesticide, both of which are used to inhibit the growth of weeds in farmland. However, the improper use of the pesticide causes pesticide residues. In turn, the environment and crop safety and even human health hazards. A new method for the detection of pesticide residues in environment and cereals by immune separation combined with surface enhanced Raman spectroscopy was established. Dendritic silver was used as Raman enhancement substrate and melamine as Raman signal to label hapten. A competitive immunoassay combined with surface enhanced Raman spectroscopy was established for the detection of pesticide residues in cereals and environment. The best scanning parameters of Raman spectrum are obtained by several experiments: scanning time is 10s, scanning times is three times, laser power is 0.5-5. The dilution times of antibody and hapten were 10 times, 100 times and 2 times respectively. The standard curve was established with the concentration gradient of 0.01ng/mL-100 渭 g/mL as the horizontal coordinate and the peak intensity of melamine signal as the vertical coordinate. The standard curve equation of atrazine is yangzhou-4871.6x 17999R2n 0.9705, and the standard curve equation of salicalum is yangzhi-7079.3x 26571r2n 0.9668 8. The standard curve equation of atrazine is Yi-4871.6x 17999N R2O 0.9705. The detection limit of the two methods is 0.01 ng / mL. The detection time, sensitivity and specificity of the two kinds of agricultural residues in the environment and cereals were short, high sensitivity and good specificity, and the standard recovery rate of the actual samples was higher. It is suitable for quantitative detection of pesticide residues.
【学位授予单位】：天津科技大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：O657.37;TQ450.263

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