红外光谱对三七及其野生近缘种亲缘关系研究

发布时间：2018-05-01 22:33

  本文选题：傅里叶变换红外光谱 + 三七　； 参考：《光谱学与光谱分析》2016年08期

【摘要】：野生近缘种在丰富种质资源以及改善栽培物种品质产量方面具有十分重要的意义和作用。采用傅里叶变换红外光谱对三七及其野生近缘种亲缘关系进行探讨,以期为三七的品种改良及种质资源开发利用提供理论基础。采集三七及其4个野生近缘种(珠子参、屏边三七、越南人参、羽叶三七)、三七总皂苷标准品红外光谱,将三七及其野生近缘种红外光谱进行自动基线校正、平滑、纵坐标归一化、二阶导数预处理,结合主成分分析、偏最小二乘判别分析及系统聚类分析进行亲缘关系研究。结果显示,三七与三七总皂苷标准品红外光谱在3 400,2 930,1 635,1 385,1 075,927 cm~(-1)等附近存在多个共有峰,推测这些共有峰强度与皂苷含量之间有一定关系;三七及其野生近缘种原始光谱较为相似,存在C-H,C=O,O—H,C—N,C—O等官能团吸收峰,不同物种的二阶导数红外光谱指纹区(1 800~500 cm~(-1))具有较大差异,如1 385和784 cm~(-1)等特征吸收峰差异明显;选取指纹区光谱数据进行主成分分析、偏最小二乘判别分析,通过比对两种方法的3D得分图,偏最小二乘判别分析对物种的区分效果优于主成分分析;前6个主成分数据的系统聚类分析表明,三七与越南人参、珠子参亲缘关系较近,与屏边三七、羽叶三七亲缘关系较远。利用傅里叶变换红外光谱结合化学计量学的方法能有效对三七及其野生近缘种进行亲缘关系分析,同时为药用植物种质资源相关研究提供借鉴与参考。
[Abstract]:Wild relative species play an important role in enriching germplasm resources and improving the quality and yield of cultivated species. The relationship between Panax notoginseng and its wild relatives was studied by Fourier transform infrared spectroscopy (FTIR) in order to provide theoretical basis for variety improvement and germplasm resources development and utilization of Panax notoginseng. The infrared spectra of Panax notoginseng and its four wild relative species (Panax japonicus, Panax notoginseng, Panax ginseng, Panax notoginseng, Panax notoginseng) were collected, and the infrared spectra of Panax notoginseng and its wild relatives were calibrated automatically and smoothly. The genetic relationship is studied by means of ordinate normalization, second derivative preprocessing, principal component analysis, partial least squares discriminant analysis and systematic cluster analysis. The results showed that there were several common peaks in the vicinity of 3400 ~ (2 930) C ~ (-1) C ~ (-1) and total saponins of Panax notoginseng and Panax notoginseng. It was inferred that there was a certain relationship between the intensity of these common peaks and the content of saponins. The original spectra of Panax notoginseng and its wild relative species were similar, and there were absorption peaks of functional groups, such as C-Haphnianthus OOOOHZOHZON C-O, and the second derivative infrared fingerprint region of different species was 1 800 ~ 500 cm ~ (-1) ~ 1 ~ (-1), and there was a great difference in the original spectra of Panax notoginseng and its wild relative species. For example, there are obvious differences in absorption peaks between 1 385 and 784 cm ~ (-1), the spectral data of fingerprint region are selected for principal component analysis, partial least squares discriminant analysis, and 3D score map of the two methods are compared. Partial least squares discriminant analysis was superior to principal component analysis in distinguishing species, and the systematic cluster analysis of the first six principal components data showed that Panax notoginseng was closely related to Panax ginseng and Radix Ginseng, and Panax notoginseng was closely related to Panax notoginseng. The phylogenetic relationship of Panax notoginseng was far away. Fourier transform infrared spectroscopy (FTIR) combined with chemometrics can effectively analyze the genetic relationship of Panax notoginseng and its wild relative species and provide reference for the study of medicinal plant germplasm resources.
【作者单位】： 云南中医学院中药学院;云南省农业科学院药用植物研究所;
【基金】：国家自然科学基金项目(81260610,81460581)资助
【分类号】：S567.236;O657.33
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本文编号：1831291