近红外光谱法快速测定乌药中乌药醚内酯和去甲异波尔定含量

发布时间：2018-05-08 02:39

本文选题：乌药 + 近红外光谱　；参考：《中国新药杂志》2017年06期

【摘要】：目的:建立乌药中乌药醚内酯和去甲异波尔定2种主要成分的近红外光谱定量模型。方法:采用高效液相色谱法测定乌药中乌药醚内酯和去甲异波尔定的含量,然后运用傅立叶变换近红外光谱技术并结合偏最小二乘法(PLS)、选择谱段为4 412~4 347,8 720~4 458 cm-1,以"二阶导数+标准正则变换"对原始光谱进行预处理建立定量分析模型;采用校正模型的相关系数(r)、校正均方根误差(RMSEC)、内部交叉验证均方根误差(RMCECV)和预测模型相关系数(r)、预测均方根误差(RMSEP)对校正模型进行评价。结果:所建立的定量校正模型中乌药醚内酯和去甲异波尔定的r分别为0.996 14和0.985 55;RMSEC分别为0.004 36和0.036 1;RMCECV分别为0.040 11和0.171 74;预测模型乌药醚内酯和去甲异波尔定的r值分别0.917 0和0.903 4;RMSEP分别为0.010 5和0.090 1。结论:该研究建立的近红外定量模型稳定性较好、预测结果较准确,可用于乌药中乌药醚内酯和去甲异波尔定2种主要成分的同步快速测定。
[Abstract]:Aim: to establish a quantitative model of etherolactone and normebordine in Radix Aconiti by near infrared spectroscopy (NIR). Methods: the content of etherolactone and normebordine were determined by HPLC. Then, using Fourier transform near infrared spectroscopy and partial least square method, a quantitative analysis model of the original spectrum was established by using "second derivative canonical transformation". The spectrum segment was 4 412 ~ (12) 4347 ~ 8 720 ~ 4 458 cm ~ (-1). The correction model is evaluated by correlation coefficient of correction model, correction of root mean square error (RMSEC), internal cross-validation of root mean square error (RMCECVV) and prediction model correlation coefficient (RMSEPs). Results: the r values of ursolide and normebordine in the model were 0.996 14 and 0.985 55 渭 m, respectively, 0.004 36 and 0.036 1 rMCECV were 0.040 11 and 0.171, respectively. The RMSEP values of 0.917 and 0.903 4g were 0.010 5 and 0.090 1, respectively. Conclusion: the NIR quantitative model established by this study has good stability and accurate prediction results. It can be used for the simultaneous and rapid determination of etherolactone and normebordine.
【作者单位】：浙江中医药大学中药炮制技术研究中心;浙江中医药大学中药饮片有限公司;
【基金】：中医药行业科研专项(201507002) 浙江省公益性技术应用研究计划资助项目(2015C33152) 浙江中医药大学重中之重一级学科开放基金项目
【分类号】：R284.1;O657.33

【相似文献】